Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide
文献类型：期刊论文
| 作者 | Fu, Xiuli1,3; Chen, Lingxin1 ; Li, Jinhua1; Lin, Meng1; You, Huiyan2; Wang, Wenhai1
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| 刊名 | BIOSENSORS & BIOELECTRONICS
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| 出版日期 | 2012-04-15 |
| 卷号 | 34期号:1页码:227-231 |
| 关键词 | Heparin Gold nanorods Graphene oxide Colorimetric detection |
| ISSN号 | 0956-5663 |
| 产权排序 | [Fu, Xiuli; Chen, Lingxin; Li, Jinhua; Lin, Meng; Wang, Wenhai] Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [You, Huiyan] Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China; [Fu, Xiuli] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 通讯作者 | Chen, LX (reprint author), Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China. lxchen@yic.ac.cn |
| 中文摘要 | A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin. |
| 英文摘要 | A novel label-free colorimetric strategy was developed for ultrasensitive detection of heparin by using the super color quenching capacity of graphene oxide (GO). Hexadecyltrimethylammonium bromide (CTAB)-stabilized gold nanorods (AuNRs) could easily self-assembly onto the surface of GO through electrostatic interaction, resulting in decrease of the surface plasmon resonance (SPR) absorption and consequent color quenching change of the AuNRs from deep to light. Polycationic protamine was used as a medium for disturbing the electrostatic interaction between AuNRs and GO. The AuNRs were prevented from being adsorbed onto the surface of GO because of the stronger interaction between protamine and GO, showing a native color of the AuNRs. On the contrary, in the presence of heparin, which was more easily to combine with protamine, the AuNRs could self-assembly onto the surface of GO, resulting in the native color disappearing of AuNRs. As the concentration of heparin increased, the color of AuNRs would gradually fade until almost colorless. The amounts of self-assembly AuNRs were proportional to the concentration of heparin, and thereby the changes in the SPR absorption and color had been used to monitor heparin levels. Under optimized conditions, good linearity was obtained in a range of 0.02-0.28 mu g/mL (R = 0.9957), and a limit of detection was 5 ng/mL. The simultaneous possession of high sensitivity and selectivity, simplicity, rapidity, and visualization enabled this sensor to be potentially applicable for ultrasensitive and rapid on-site detection toward trace heparin. (c) 2012 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics; Biotechnology & Applied Microbiology; Chemistry; Electrochemistry; Science & Technology - Other Topics |
| 研究领域[WOS] | Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics |
| 关键词[WOS] | SELECTIVE DETECTION ; QUANTIFICATION ; CHROMATOGRAPHY ; NANOPARTICLES ; GROWTH ; PROBES ; DRUGS |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[20975089, 21105117, 20875088]; Chinese Academy of Sciences[KZCX2-EW-206] |
| 原文出处 | http://www.sciencedirect.com/science/journal/09565663 |
| 语种 | 英语 |
| WOS记录号 | WOS:000302886500035 |
| 公开日期 | 2012-06-13 |
| 源URL | [http://ir.yic.ac.cn/handle/133337/5514]  |
| 专题 | 烟台海岸带研究所_山东省海岸带环境工程技术研究中心 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Coastal Zone Environm Proc, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China 2.Dalian Univ Technol, Environm & Chem Engn Coll, Dalian 116622, Peoples R China 3.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Fu, Xiuli,Chen, Lingxin,Li, Jinhua,et al. Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide[J]. BIOSENSORS & BIOELECTRONICS,2012,34(1):227-231. |
| APA | Fu, Xiuli,Chen, Lingxin,Li, Jinhua,Lin, Meng,You, Huiyan,&Wang, Wenhai.(2012).Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide.BIOSENSORS & BIOELECTRONICS,34(1),227-231. |
| MLA | Fu, Xiuli,et al."Label-free colorimetric sensor for ultrasensitive detection of heparin based on color quenching of gold nanorods by graphene oxide".BIOSENSORS & BIOELECTRONICS 34.1(2012):227-231. |
入库方式： OAI收割
来源：烟台海岸带研究所
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