A Label-Free, Quadruplex-Based Functional Molecular Beacon (LFG4-MB) for Fluorescence Turn-On Detection of DNA and Nuclease
文献类型：期刊论文
| 作者 | Hu D ; Huang ZZ ; Pu F ; Ren JS ; Qu XG |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2011 |
| 卷号 | 17期号:5页码:1635-1641 |
| ISSN号 | 0947-6539 |
| 关键词 | EXCISION-REPAIR PROBES OLIGONUCLEOTIDES GLYCOSYLASE SEQUENCE HYBRIDIZATION SELECTIVITY MACHINE ASSAY |
| 通讯作者 | ren js |
| 中文摘要 | we demonstrate a novel concept for the construction of a label-free, quadruplex-based functional molecular beacon (lfg4-mb) by using g-quadruplex motif as a substitute for watson crick base pairing in the mb stem and a specific g-quadruplex binder, n-methyl mesoporphyrin ix (nmm) as a reporter. it shows high sensitivity in assays for udg activity/inhibition and detection of dna sequence based on the unique fluorescence increase that occurs as a result of the strong interaction between nmm and the folded quadruplex upon removal of uracil by udg or displacement of block sequence by target dna. the lfg4-mb is simple in design, fast in operation and could be easily transposesd to other biological relevant target analysis by simply changing the recognition portion. the lfg4-mb does not require any chemical modification for dna, which offers the advantages of simplicity and cost efficiency and obviates the possible interference with the affinity and specificity of the mb as well as the kinetic behavior of the catalysts caused by the bulky fluorescent groups. more importantly, the lfg4-mb offers great extent of freedom to tune the experimental conditions for the general applicability in bioanalysis. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000287555600029 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/45056]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Hu D,Huang ZZ,Pu F,et al. A Label-Free, Quadruplex-Based Functional Molecular Beacon (LFG4-MB) for Fluorescence Turn-On Detection of DNA and Nuclease[J]. chemistry-a european journal,2011,17(5):1635-1641. |
| APA | Hu D,Huang ZZ,Pu F,Ren JS,&Qu XG.(2011).A Label-Free, Quadruplex-Based Functional Molecular Beacon (LFG4-MB) for Fluorescence Turn-On Detection of DNA and Nuclease.chemistry-a european journal,17(5),1635-1641. |
| MLA | Hu D,et al."A Label-Free, Quadruplex-Based Functional Molecular Beacon (LFG4-MB) for Fluorescence Turn-On Detection of DNA and Nuclease".chemistry-a european journal 17.5(2011):1635-1641. |
入库方式： OAI收割
来源：长春应用化学研究所
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