G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element
文献类型：期刊论文
| 作者 | Du Y ; Li BL ; Guo SJ ; Zhou ZX ; Zhou M ; Wang EK ; Dong SJ |
| 刊名 | analyst
 |
| 出版日期 | 2011 |
| 卷号 | 136期号:3页码:493-497 |
| 关键词 | DNA PEROXIDASE SENSOR MICROSPHERES APTAMERS LIGANDS PROBE |
| ISSN号 | 0003-2654 |
| 通讯作者 | wang ek |
| 中文摘要 | the appearance of the aptamer provides good recognition elements for small molecules, especially for drugs. in this work, by combining the advantages of magnetic nanoparticles (mnps) with colorimetric drug detection using hemin-g-quadruplex complex as the sensing element, we report a simple and sensitive dnazyme-based colorimetric sensor for cocaine detection in a 3,3,5,5-tetramethylbenzidine sulfate (tmb)-h(2)o(2) reaction system. the whole experimental processes are simplified. cocaine aptamer fragments, sh-c2, are covalently labeled onto the amine-functionalized mnps. when the target cocaine and another cocaine aptamer fragments (c1) grafted with g-riched strand ag4 (i.e. c1-ag4) are present simultaneously, the c2 layer on mnps hybridizes partly with c1-ag4 to bind the cocaine. the c1-ag4 can be combinded with hemin to form dnazyme which can effectively catalyze the h(2)o(2)-mediated oxidation of tmb, giving rise to a change in solution color. importantly, using mnps as the separation and amplification elements could effectively reduce the background signal and the interference from the real samples. a linear response from 0.1 mu m to 20 mu m is obtained for cocaine and a detection limit of 50 nm is achieved, which provides high sensitivity and selectivity to detect cocaine. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000286326800012 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/45073]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Du Y,Li BL,Guo SJ,et al. G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element[J]. analyst,2011,136(3):493-497. |
| APA | Du Y.,Li BL.,Guo SJ.,Zhou ZX.,Zhou M.,...&Dong SJ.(2011).G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element.analyst,136(3),493-497. |
| MLA | Du Y,et al."G-Quadruplex-based DNAzyme for colorimetric detection of cocaine: Using magnetic nanoparticles as the separation and amplification element".analyst 136.3(2011):493-497. |
入库方式： OAI收割
来源：长春应用化学研究所
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