Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay
文献类型：期刊论文
| 作者 | Li XM ; Gao JQ ; Liu DJ ; Wang ZX |
| 刊名 | analyst
 |
| 出版日期 | 2011 |
| 卷号 | 136期号:20页码:4301-4307 |
| 关键词 | CAPILLARY AFFINITY ELECTROPHORESIS GOLD NANOPARTICLE PROBES CONCANAVALIN-A CELL-INTERACTIONS N-GLYCANS BINDING SURFACE LECTINS RECOGNITION LIGANDS |
| ISSN号 | 0003-2654 |
| 通讯作者 | liu dj |
| 中文摘要 | here, a three-dimensional (3d) carbohydrate microarray-based plasmon resonance light scattering (rls) assay has been established for studying carbohydrate-lectin binding with high selectivity. the 3d carbohydrate microarray is fabricated by immobilizing amino-modified carbohydrates on the home-made fourth-generation (g4) nh(2)-terminated poly(amidoamine) dendrimers (pamam)-modified substrate. after marking the carbohydrate-lectin binding events by 13 nm peptide-stabilized gold nanoparticles through the biotin-avidin reaction, the 3d microarray can be directly detected by the rls scanner without the conventional silver enhancement step. the well defined recognition systems: three monosaccharides (man-alpha, glc-alpha and gal-beta) with two lectins (con a and rca 120), have been chosen here to establish the rls assay, respectively. quantitative determination of the surface dissociation constants (k(d,surf)) for surface carbohydrates and lectins has been achieved. in addition, inhibition values (i.e. the inhibition constants (k(i)) and the concentrations of inhibitors required to produce 50% inhibition (ic(50))) for inhibitors in solution are also demonstrated by the saccharide competing assays. |
| 收录类别 | SCI收录期刊论文 |
| 语种 | 英语 |
| WOS记录号 | WOS:000295334000032 |
| 公开日期 | 2012-06-12 |
| 源URL | [http://ir.ciac.jl.cn/handle/322003/45130]  |
| 专题 | 长春应用化学研究所_长春应用化学研究所知识产出_期刊论文 |
| 推荐引用方式 GB/T 7714 | Li XM,Gao JQ,Liu DJ,et al. Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay[J]. analyst,2011,136(20):4301-4307. |
| APA | Li XM,Gao JQ,Liu DJ,&Wang ZX.(2011).Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay.analyst,136(20),4301-4307. |
| MLA | Li XM,et al."Studying the interaction of carbohydrate-protein on the dendrimer-modified solid support by microarray-based plasmon resonance light scattering assay".analyst 136.20(2011):4301-4307. |
入库方式： OAI收割
来源：长春应用化学研究所
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