A study on differentially expressed gene screening of Chrysanthemum plants under sound stress
文献类型:期刊论文
作者 | Shao, HB (Shao Hongbo)1,2,3 ; Li, B (Li Biao)4,5 ; Wang, BC (Wang Bochu)4,5 ; Tang, K (Tang Kun)4,5 ; Liang, YL (Liang Yilong)4 |
刊名 | COMPTES RENDUS BIOLOGIES
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出版日期 | 2008 |
卷号 | 331期号:5页码:329-333 |
关键词 | DDRT-PCR (Differentially Display Reverse Transcription-Polymerase Chain Reaction) northern dot hybridization sound stress physiological response Chrysanthemum |
通讯作者 | Shao, HB |
英文摘要 | Environmental stress can induce differential expression of genes of flower plants. It had been found that sound stimulation had an obvious effect on the growth and development of flower plants, but it is not reported on the differentially expressed genes and their expressing characteristics under sound stimulation. This is one of the few reports in terms of using the DDRT-PCR technique for screening the differentially expressed cDNA fragments responding to sound-wave stress on Chrysanthemum. Six differentially expressed cDNA fragments were obtained. Molecular weight of fragments was from 200 to 600 bp, respectively. Among differential fragments acquired, three of them (SA3, SG7-1, and CA2) were found to be positive fragments by northern dot hybridization, whose molecular weight are 270, 580 and 370 bp, respectively. SA3 was differentially expressed and SG7-1 was preferably expressed, while CA2 was restrained by the sound wave. These results indicated that expression of some genes was turned on, meanwhile the stress restrained some genes from expression under the mode of sound-stress stimulation. |
收录类别 | SCI |
语种 | 英语 |
公开日期 | 2012-06-14 |
源URL | [http://ir.iswc.ac.cn/handle/361005/4852] ![]() |
专题 | 水土保持研究所_水保所知识产出(1956-2013) |
推荐引用方式 GB/T 7714 | Shao, HB ,Li, B ,Wang, BC ,et al. A study on differentially expressed gene screening of Chrysanthemum plants under sound stress[J]. COMPTES RENDUS BIOLOGIES,2008,331(5):329-333. |
APA | Shao, HB ,Li, B ,Wang, BC ,Tang, K ,&Liang, YL .(2008).A study on differentially expressed gene screening of Chrysanthemum plants under sound stress.COMPTES RENDUS BIOLOGIES,331(5),329-333. |
MLA | Shao, HB ,et al."A study on differentially expressed gene screening of Chrysanthemum plants under sound stress".COMPTES RENDUS BIOLOGIES 331.5(2008):329-333. |
入库方式: OAI收割
来源:水土保持研究所
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