A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings
文献类型：期刊论文
| 作者 | Li, Jing1,2,3 ; Zhao, Pei-Ji1; Ma, Chang-Le3; Zeng, Ying1
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| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2012 |
| 卷号 | 13期号:1页码:540-551 |
| 关键词 | Adelostemma gracillimum lipoxygenase chitosan trihydroxyl octadecenoic acid functional expression AgLOX1 |
| ISSN号 | 1661-6596 |
| 通讯作者 | Zeng, Y (reprint author), Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China, lijing@mail.kib.ac.cn ; plenty@mail.kib.ac.cn ; machangle@sina.com ; biochem@mail.kib.ac.cn |
| 英文摘要 | Oxylipins generated by the lipoxygenase (LOX) pathway play an important role in plant defense against biotic and abiotic stress. In chitosan-treated Adelostemma gracillimum seedlings, obvious accumulation of 9-LOX-derived oxylipins, namely 9,10,11-trihydroxy-12-octadecenoic acid, was detected. Using degenerate primers, a LOX-specific fragment putatively encoding LOX was obtained by RT-PCR, and a 2.9-kb full-length cDNA named AgLOX1 was isolated by RACE from chitosan-induced A. gracillimum seedlings. Genomic Southern analysis implied that there was only one copy of AgLOX1 in the A. gracillimum genome. AgLOX1 was expressed in Escherichia coli and the recombinant protein was partially purified. The enzyme converted linoleic and linolenic acids almost exclusively to their 9-hydroperoxides. AgLOX1 encoded a 9-lipoxygenase. Northern blot analysis indicated that chitosan-induced AgLOX1 transcript accumulation peaked at 8 h after initiation of treatment, whereas trihydroxy derivatives accumulation was highest at 24 h after elicitation. Results showed that chitosan-induced AgLOX1 encoded a 9-lipoxygenase potentially involved in the defense response through 9-LOX pathway leading to biosynthesis of antimicrobial compounds in A. gracillimum seedlings. |
| 学科主题 | Chemistry, Multidisciplinary |
| 类目[WOS] | Chemistry, Multidisciplinary |
| 研究领域[WOS] | Chemistry |
| 关键词[WOS] | METHYL JASMONATE ; LIPOXYGENASE PATHWAY ; PLANT LIPOXYGENASE ; ABSCISIC-ACID ; BIOSYNTHESIS ; OXYLIPINS ; CUCUMBER ; LEAVES ; POTATO |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of Yunnan Province[2009ZC085M]; State Key Laboratory of Phytochemistry and Plant Resources in West China[P2009-ZZ02] |
| 语种 | 英语 |
| WOS记录号 | WOS:000300184800036 |
| 公开日期 | 2012-06-07 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/10387]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources W China, Kunming 650204, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China 3.SW Forestry Univ, Life Sci Coll, Kunming 650224, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,et al. A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2012,13(1):540-551. |
| APA | Li, Jing,Zhao, Pei-Ji,Ma, Chang-Le,&Zeng, Ying.(2012).A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,13(1),540-551. |
| MLA | Li, Jing,et al."A Chitosan Induced 9-Lipoxygenase in Adelostemma gracillimum Seedlings".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 13.1(2012):540-551. |
入库方式： OAI收割
来源：昆明植物研究所
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