Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides
文献类型：期刊论文
| 作者 | Tang, Zhuzhao ; Chen, Haode ; Song, Shiping(宋世平） ; Fan, Chunhai（樊春海） ; Zhang, Dabing ; Wu, Aibo |
| 刊名 | JOURNAL OF AOAC INTERNATIONAL
 |
| 出版日期 | 2011 |
| 卷号 | 94期号:1页码:307 |
| ISSN号 | 1060-3271 |
| 英文摘要 | Recombinant Drosophila melanogaster acetylcholinesterase (R-DmAChE), multiwalled carbon nanotubes (MWCNTs), and Prussian blue have been combined for development of a three-electrode biosensor with more rapid responses and higher stability than in our previous study. A new disposable screen-printed electrode (SPE) was developed for rapid detection of organophosphate and carbamate pesticides. After optimization, 10 mu g MWCNT and 5 mu L enzyme immobilization solution consisting of 0.2% glutaraldehyde, 0.1% Nafion (R), 0.2% bovine serum albumin, 0.1 g/L MWCNT, and 1.5 mU R-DmAChE were fixed on each of the R-DmAChE/MWCNT SPEs. The LOD of this biosensor was 0.5 mu g/L for pesticide standards of dichlorvos (DDV) and carbofuran. The performance of this biosensor was tested for vegetable and water samples at various spiked levels, and good stability and sensitivity were found. The obtained recoveries were from 82.6 to 110.5% for DDV at levels of 0.5-5 mu g/L and 73.4 to 118.4% for carbofuran at 1-10 mu g/L in lake and sea water samples, demonstrating that the proposed approach is an alternative means for rapid detection of pesticide residues and contaminants in food safety and environmental monitoring. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287222000035 |
| 公开日期 | 2012-07-04 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/9492]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Tang, Zhuzhao,Chen, Haode,Song, Shiping,et al. Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides[J]. JOURNAL OF AOAC INTERNATIONAL,2011,94(1):307. |
| APA | Tang, Zhuzhao,Chen, Haode,Song, Shiping,Fan, Chunhai,Zhang, Dabing,&Wu, Aibo.(2011).Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides.JOURNAL OF AOAC INTERNATIONAL,94(1),307. |
| MLA | Tang, Zhuzhao,et al."Disposable Screen-Printed Electrode Coupled with Recombinant Drosophila melanogaster Acetylcholinesterase and Multiwalled Carbon Nanotubes for Rapid Detection of Pesticides".JOURNAL OF AOAC INTERNATIONAL 94.1(2011):307. |
入库方式： OAI收割
来源：上海应用物理研究所
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