Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure
文献类型：期刊论文
| 作者 | Ge ZhiLei ; Pei Hao ; Wang LiHua（王丽华） ; Song ShiPing（宋世平） ; Fan ChunHai（樊春海） |
| 刊名 | SCIENCE CHINA-CHEMISTRY
 |
| 出版日期 | 2011 |
| 卷号 | 54期号:8页码:1273 |
| ISSN号 | 1674-7291 |
| 英文摘要 | An electrochemical assay for single nucleotide polymorphisms (SNPs) genotyping is reported. Although electrochemical method is sensitive for DNA detection on surfaces, the ability of surface assay to precisely recognize DNA hybridization event is sacrificed to some extent due to the crowded confined surfaces environments that disfavor DNA hybridization. In the present study, we employed branched tetrahedron structure probes (TSPs) to replace regular linear single stranded DNA capture probes that were immobilized on solid surfaces. This three-dimensional DNA nanostructure lowers the density of immobilized DNA probes on confined surfaces, providing a hybridization environment that is similar to homogenous solution. This TSP-based electrochemical assay reveals excellent performance for SNPs genotyping with concentration as low as 1 nM. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000294062100013 |
| 公开日期 | 2012-07-04 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/9503]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Ge ZhiLei,Pei Hao,Wang LiHua,et al. Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure[J]. SCIENCE CHINA-CHEMISTRY,2011,54(8):1273. |
| APA | Ge ZhiLei,Pei Hao,Wang LiHua,Song ShiPing,&Fan ChunHai.(2011).Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure.SCIENCE CHINA-CHEMISTRY,54(8),1273. |
| MLA | Ge ZhiLei,et al."Electrochemical single nucleotide polymorphisms genotyping on surface immobilized three-dimensional branched DNA nanostructure".SCIENCE CHINA-CHEMISTRY 54.8(2011):1273. |
入库方式： OAI收割
来源：上海应用物理研究所
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