Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy
文献类型：期刊论文
| 作者 | Chen, Guangcun ; Chen, Xincai ; Yang, Yuanqiang ; Hay, Anthony G. ; Yu, Xiaohan（余笑寒） ; Chen, Yingxu |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2011 |
| 卷号 | 77期号:14页码:4719 |
| ISSN号 | 0099-2240 |
| 英文摘要 | The spatial and temporal distribution of metals in unsaturated Pseudomonas putida CZ1 biofilms was determined using synchrotron-based X-ray fluorescence microscopy (XRF). It was found that Fe, Mn, and Ca were mainly distributed near the air-biofilm interface of a biofilm grown on 40 mM citrate, while there were two Fe-, Mn-, and Ca-rich layers within a biofilm grown on 10 mM citrate. The sorption of copper by biofilm grown in medium containing 10 mM citrate was rapid, with copper being found throughout the biofilm after only 1 h of exposure. Copper initially colocalized with Fe and Mn element layers in the biofilm and then precipitated in a 40-mu m-thick layer near the air-biofilm interface when exposed for 12 h. Cu K-edge X-ray absorption near edge structure (XANES) analysis revealed that Cu was primarily bound with citrate within the biofilm, and the precipitate formed in the biofilm exposed to copper for 12 h was most similar to copper phosphate. LIVE/DEAD staining revealed that cells at the biofilm-membrane interface were mostly alive even when the copper concentration reached 80.5 mg copper g(-1) biomass. This suggests that the biofilm matrix provided significant protection for cells in this area. These results significantly improve our understanding of metal acquisition, transportation, and immobilization in unsaturated biofilm systems. |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292510400003 |
| 公开日期 | 2012-07-04 |
| 源URL | [http://ir.sinap.ac.cn/handle/331007/9591]  |
| 专题 | 上海应用物理研究所_中科院上海应用物理研究所2011-2017年 |
| 推荐引用方式 GB/T 7714 | Chen, Guangcun,Chen, Xincai,Yang, Yuanqiang,et al. Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2011,77(14):4719. |
| APA | Chen, Guangcun,Chen, Xincai,Yang, Yuanqiang,Hay, Anthony G.,Yu, Xiaohan,&Chen, Yingxu.(2011).Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,77(14),4719. |
| MLA | Chen, Guangcun,et al."Sorption and Distribution of Copper in Unsaturated Pseudomonas putida CZ1 Biofilms as Determined by X-Ray Fluorescence Microscopy".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 77.14(2011):4719. |
入库方式： OAI收割
来源：上海应用物理研究所
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