The first insight into the Taxus genome via fosmid library construction and end sequencing
文献类型：期刊论文
| 作者 | Hao, DaCheng1; Yang, Ling2; Xiao, PeiGen3 |
| 刊名 | molecular genetics and genomics
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 285期号:3页码:197-205 |
| 关键词 | Taxus mairei Fosmid genomic library Fosmid end sequences Repetitive sequences Comparative genomics |
| ISSN号 | 待补充 |
| 产权排序 | 2,2 |
| 通讯作者 | 杨凌 |
| 中文摘要 | the first insight into the taxus genome via fosmid library construction and end sequencing |
| 英文摘要 | taxus mairei is a critically endangered and commercially important cultured medicinal gymnosperm in china and forms an important medicinal resource, but the research of its genome is absent. in this study, we constructed a t. mairei fosmid library and analyzed the fosmid end sequences to provide a preliminary assessment of the genome. the library consists of one million clones with an average insert size of about 39 kb, amounting to 3.9 genome equivalents. fosmid stability assays indicate that t. mairei dna was stable during propagation in the fosmid system. end sequencing of both 5' and 3' ends of 968 individual clones generated 1,923 sequences after trimming, with an average sequence length of 839 bp. blastn searches of the nr and est databases of genbank and blastx searches of the nr database resulted in 560 (29.1%) significant hits (e < e(-5)). repetitive sequences analysis revealed that 20.8% of end sequences are repetitive elements, which were composed of retroelements, dna transposons, satellites, simple repeats, and low complexity sequences. the distribution pattern of various repeat types was found to be more similar to the gymnosperm pinus and picea than to the monocot and dicot. the satellites of t. mairei were significantly longer than those of p. taeda and p. glauca. the tetra-nucleotide repeats of t. mairei were much longer than those of p. glauca and p. taeda. the fosmid library and the fosmid end sequences, for the first time, will serve as a useful resource for large-scale genome sequencing, physical mapping, ssr marker development and positional cloning, and provide a better understanding of the taxus genome. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 研究领域[WOS] | biochemistry & molecular biology ; genetics & heredity |
| 关键词[WOS] | structural variation ; molecular evolution ; initial analysis ; dna ; chloroplast ; identification ; paclitaxel ; taxaceae ; genes ; rice |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287665100002 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115183]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Dalian Jiaotong Univ, Inst Biotechnol, Dalian 116028, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 3.Chinese Acad Med Sci, Inst Med Plant Dev, Beijing 100193, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hao, DaCheng,Yang, Ling,Xiao, PeiGen. The first insight into the Taxus genome via fosmid library construction and end sequencing[J]. molecular genetics and genomics,2011,285(3):197-205. |
| APA | Hao, DaCheng,Yang, Ling,&Xiao, PeiGen.(2011).The first insight into the Taxus genome via fosmid library construction and end sequencing.molecular genetics and genomics,285(3),197-205. |
| MLA | Hao, DaCheng,et al."The first insight into the Taxus genome via fosmid library construction and end sequencing".molecular genetics and genomics 285.3(2011):197-205. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。