Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins
文献类型：期刊论文
| 作者 | Wang, Chunli1,2; Ye, Mingliang1; Han, Guanghui1,2; Chen, Rui1,2; Zhang, Manyu1,2; Jiang, Xinning1,2; Cheng, Kai1,2; Wang, Fangjun1,2; Zou, Hanfa1 |
| 刊名 | proteomics
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 11期号:17页码:3578-3581 |
| 关键词 | MS Protein kinases Proteomics methods Selective isolation Targeted analysis Technology |
| ISSN号 | 1615-9853 |
| 产权排序 | 1,1 |
| 通讯作者 | 邹汉法 ; 叶明亮 |
| 中文摘要 | enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins |
| 英文摘要 | multiple residues with consensus sequence, i.e. motif, on proteins are closely related to protein function. however, there is no effective method for targeted analysis of such proteins. the challenge for analysis of these classes of proteins by ms is how to selectively enrich peptides containing consensus sequence from protein digest. although enrichment of peptides containing one type of amino acid residue was successfully achieved by chemically labeling followed by chromatographic isolation, however, it is almost impossible to label and isolate signature peptides containing multiple residues with consensus sequence by chemical approach. herein, we developed an enzymatic approach based on the specific recognition between enzyme and its substrates to enrich such peptides. this approach was realized by modification of a residue in the consensus sequence via enzyme that can recognize the sequence followed by the isolation of the modified peptides. camp-dependent protein kinase was used to validate this approach and 168 peptides containing consensus motif were identified with selectivity of 67.2%. those peptides resulted in the identification of 88 proteins with consensus sequence from serum sample. as this motif-oriented peptide enrichment approach allows targeted analysis of a subset of proteins with consensus sequence, it will have broad application in biological studies. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | mass-spectrometry ; proteome analysis ; tryptic peptides ; identification ; residues ; tool |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295217400014 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115262]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Chunli,Ye, Mingliang,Han, Guanghui,et al. Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins[J]. proteomics,2011,11(17):3578-3581. |
| APA | Wang, Chunli.,Ye, Mingliang.,Han, Guanghui.,Chen, Rui.,Zhang, Manyu.,...&Zou, Hanfa.(2011).Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins.proteomics,11(17),3578-3581. |
| MLA | Wang, Chunli,et al."Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis of proteins".proteomics 11.17(2011):3578-3581. |
入库方式： OAI收割
来源：大连化学物理研究所
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