Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy
文献类型：期刊论文
| 作者 | Wang, Fangjun; Song, Chunxia; Cheng, Kai; Jiang, Xinning; Ye, Mingliang; Zou, Hanfa |
| 刊名 | analytical chemistry
 |
| 出版日期 | 2011-11-01 |
| 卷号 | 83期号:21页码:8078-8085 |
| ISSN号 | 0003-2700 |
| 产权排序 | 1,1 |
| 通讯作者 | 邹汉法 |
| 中文摘要 | perspectives of comprehensive phosphoproteome analysis using shotgun strategy |
| 英文摘要 | protein phosphorylation is a ubiquitous post-translational modification that regulates almost all cellular processes. the analysis, of protein phosphorylation is challenging due to the high dynamic range and low abundance natures of the analyte. shotgun based proteomics has emerged as a very useful platform to achieve a comprehensive phosphoproteome analysis in considerable depth. in the past few years, significant breakthroughs on the large scale phosphorylation analysis have been witnessed along with the great development of related technologies. the combination of effective enrichment materials, refined analysis workflows, new type of powerful mass spectrometers, and sophisticated bioinformatic tools greatly boost the performance of. comprehensive phosphoproteome analysis. in this perspective, we briefly reviewed recent technological developments on the enrichment materials, prefractionation workflows, and different mass spectrometry fragmentation modes as well as software tools for phosphoproteome identification and quantification. then, we described the current challenges and potential directions for the future of comprehensive phosphoproteome analysis. we also provide perspectives on how to further improve the performance of related analysis methods and technologies. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | tandem mass-spectrometry ; maldi-tof ms ; protein identification technology ; ion affinity-chromatography ; highly selective enrichment ; quantitative phosphoproteomics ; phosphorylated peptides ; site localization ; titanium-dioxide ; phosphopeptide identifications |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296225300008 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115300]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Fangjun,Song, Chunxia,Cheng, Kai,et al. Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy[J]. analytical chemistry,2011,83(21):8078-8085. |
| APA | Wang, Fangjun,Song, Chunxia,Cheng, Kai,Jiang, Xinning,Ye, Mingliang,&Zou, Hanfa.(2011).Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy.analytical chemistry,83(21),8078-8085. |
| MLA | Wang, Fangjun,et al."Perspectives of Comprehensive Phosphoproteome Analysis Using Shotgun Strategy".analytical chemistry 83.21(2011):8078-8085. |
入库方式： OAI收割
来源：大连化学物理研究所
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