Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Wang, Hui1,2; Duan, Jicheng1,2; Xu, Hongjiu3; Zhao, Liang1,2; Liang, Yu1,2; Shan, Yichu1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 34期号:16-17页码:2113-2121 |
| ISSN号 | 待补充 |
| 关键词 | Immobilized zirconium ion affinity chromatography Monolith Phosphopeptide Photografting Photopolymerization Selective enrichment |
| 通讯作者 | 张丽华 |
| 产权排序 | 1,1 |
| 中文摘要 | monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides |
| 英文摘要 | to meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (zr4+-imac) monolith was prepared by combining uv-initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. hydrophilic poly(2-hydroxyethyl methacrylate (hema)-co-ethylene dimethacrylate (edma)) monolithic support was prepared under uv irradiation at the wavelength of 365 nm with monomer hema, crosslinker edma and 2,2-dimethoxy-2-phenylacetophenone as photoinitiator in 1-decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. to introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. the grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post-modification. this new method for the preparation of zr4+-imac monolith simplifies the optimization of monolith preparation and avoids the time-consuming chemical modification process. additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. after optimization, the microchip-based zr4+-imac monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix-assisted laser desorption ionization mass spectrometry detection. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | metal affinity-chromatography ; capillary liquid-chromatography ; porous polymer monoliths ; phosphoproteome analysis ; phosphorylated peptides ; mass-spectrometry ; microfluidic chips ; surface-chemistry ; columns ; electrochromatography |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295189400019 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115303]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China 3.Dalian Med Univ, Affiliated Hosp 1, Dalian, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Hui,Duan, Jicheng,Xu, Hongjiu,et al. Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides[J]. journal of separation science,2011,34(16-17):2113-2121. |
| APA | Wang, Hui.,Duan, Jicheng.,Xu, Hongjiu.,Zhao, Liang.,Liang, Yu.,...&Zhang, Yukui.(2011).Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides.journal of separation science,34(16-17),2113-2121. |
| MLA | Wang, Hui,et al."Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides".journal of separation science 34.16-17(2011):2113-2121. |
入库方式： OAI收割
来源：大连化学物理研究所
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