Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Zhang, Liyuan1,2; Wang, Hui1,2; Liang, Zhen1; Yang, Kaiguang1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011-09-01 |
| 卷号 | 34期号:16-17页码:2122-2130 |
| 关键词 | Immobilized metal affinity chromatography Monolithic column Phosphopeptides Ti(4+) Zr(4+) |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides |
| 英文摘要 | the selective enrichment of phosphopeptides with good reproducibility is vital for the indepth study of the phosphoproteome. herein, we presented a novel method to prepare monolithic ti(4+) or zr(4+) immobilized metal affinity chromatography (imac) columns. since succinimide was of high reaction activity with nitrilotriacetic acid (nta) than traditional epoxy group, through the reaction of succinimide group on the monolithic matrix with nitrilotriacetic acid, the preparation of monolithic imac columns became facile. by the developed new method, not only the time required for ti(4+) or zr(4+) immobilization could be shortened from 10 to 1 h, but also the rsd of obtained phosphopeptide peak area was below 13%, even with imac columns prepared in different batches (n = 3). with such monolithic ti(4+)- and zr(4+)-imac columns, ten and seven phosphopeptides were effectively identified from the digests of a mixture of beta-casein and bsa, even with the molar ratio as low as 1:100, respectively. the phosphopeptide recovery was over 73%, and the loading capacity was over 0.8 mu mol/ml. compared with commercial imac beads, the monolithic imac columns provide outstanding reproducibility, good selectivity, large loading capacity, and high recovery, which demonstrates that such monolithic imac columns might facilitate the in-depth analysis of phospho-proteomes. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; phosphoproteome analysis ; protein-phosphorylation ; mitochondrial phosphoproteome ; microspheres ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295189400020 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115304]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Liyuan,Wang, Hui,Liang, Zhen,et al. Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides[J]. journal of separation science,2011,34(16-17):2122-2130. |
| APA | Zhang, Liyuan,Wang, Hui,Liang, Zhen,Yang, Kaiguang,Zhang, Lihua,&Zhang, Yukui.(2011).Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides.journal of separation science,34(16-17),2122-2130. |
| MLA | Zhang, Liyuan,et al."Facile preparation of monolithic immobilized metal affinity chromatography capillary columns for selective enrichment of phosphopeptides".journal of separation science 34.16-17(2011):2122-2130. |
入库方式： OAI收割
来源：大连化学物理研究所
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