High sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization
文献类型：期刊论文
| 作者 | Qiao, Xiaoqiang1,2; Sun, Liangliang1,2; Wang, Li3; Liang, Yu1,2; Zhang, Lihua1; Shan, Yichu1; Peng, Xiaojun3; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2011-05-15 |
| 卷号 | 879期号:17-18页码:1439-1443 |
| 关键词 | In situ fluorescence derivatization Hollow fiber membrane interface Preconcentration Low abundance proteins |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | high sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization |
| 英文摘要 | a novel device, composed of a syringe pump for sample loading, a hydrophilic hollow fiber membrane interface for protein concentration and small molecules removal, and a centrifugation tube for buffer exchange, was designed for protein preconcentration and in situ fluorescence derivatization. with the outlet of the interface blocked, denatured proteins were continually introduced. restricted by the membrane with the molecular weight cutoff (mwco) of 3000 da, proteins were concentrated within the membrane. however, denaturant and other small molecules, which might affect the further fluorescence derivatization, were driven out of the membrane. then, the membrane with proteins restricted inside was directly put into the fluorescence derivatization buffer. here, the water-soluble sulfo-3h-indocyanine dye, the active n-hydroxysuccinimide of 3h-indolium,1-(5-carboxypentyl)-2[3-[1-(5-carboxypentyl)-1,3-dihydro-3,3-dimethyl-5-sulfo-2h-indol-2-ylidene]-1-propenyl]-3,3-dimethy1-5-sulfo-,monopotassium salt (sw-cy3-nhs). synthesized in our lab. was used for protein labeling. by such a method, the detection sensitivity of bovine serum albumin (bsa) was improved by nearly 200 folds, compared to that obtained by direct in-solution derivatization. through the derivatization of a fraction of e. colt protein separated by reversed phase hplc, proteins with low concentration were efficiently labeled, which indicated the potential merit of the developed method for the high sensitive detection of low abundance proteins. (c) 2010 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | sodium dodecyl-sulfate ; performance liquid-chromatography ; capillary gel-electrophoresis ; tandem mass-spectrometry ; native proteins ; amino-acids ; preconcentration ; separation ; identification ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291131900027 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115307]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Dalian Univ Technol, State Key Lab Fine Chem, Dalian 116012, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Xiaoqiang,Sun, Liangliang,Wang, Li,et al. High sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(17-18):1439-1443. |
| APA | Qiao, Xiaoqiang.,Sun, Liangliang.,Wang, Li.,Liang, Yu.,Zhang, Lihua.,...&Zhang, Yukui.(2011).High sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(17-18),1439-1443. |
| MLA | Qiao, Xiaoqiang,et al."High sensitive protein detection by hollow fiber membrane interface based protein enrichment and in situ fluorescence derivatization".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.17-18(2011):1439-1443. |
入库方式： OAI收割
来源：大连化学物理研究所
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