On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis
文献类型：期刊论文
| 作者 | Wang, Tingting1,2; Ma, Junfeng1; Wu, Shuaibin1,2; Sun, Liangliang1,2; Yuan, Huiming1; Zhang, Lihua1; Liang, Zhen1; Zhang, Yukui1 |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 879期号:11-12页码:804-810 |
| 关键词 | Two-dimensional capillary electrophoresis Monolithic immobilized pH gradient-based capillary isoelectric focusing Capillary zone electrophoresis Partially etched porous interface Protein |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | on-line combination of monolithic immobilized ph gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis |
| 英文摘要 | an integrated platform consisting of monolithic immobilized ph gradient-based capillary isoelectric focusing (m-ipg cief) and capillary zone electrophoresis (cze) coupled by a partially etched porous interface was established. since carrier ampholytes (cas) were immobilized on monolith in m-ipg cief to form a stable ph gradient, subsequent depletion of cas at the interface to prevent the interference on cze separation and detection were avoided. moreover, a partially etched porous capillary column, which was facile for fabrication and durable for operation, was exploited as the interface to combine m-ipg cief and cze. the rsd values in terms of the migration time for m-ipg cief separation, transfer protein from the first dimension to the second dimension, and cze separation, were 2.4%, 3.9% and 2.3%, respectively. with a 6-protein mixture as the sample, two-dimensional capillary electrophoresis (2d-ce) separation was successfully completed within 116 min, yielding a peak capacity of similar to 200 even with minute sample amount down to 5.0 mu g/ml. the limit of detection was 0.2 mu g/ml. in addition, proteins extracted from milk were used to test the performance of such a 2d-ce separation platform. we expect that such a novel 20-ce system would provide a promising tool for protein separation with high throughput and high peak capacity. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | 2-dimensional separation system ; mass-spectrometry ; gel-electrophoresis ; proteomics ; column ; joint ; identification ; chromatography ; integration ; peptides |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000289382700018 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115309]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Tingting,Ma, Junfeng,Wu, Shuaibin,et al. On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(11-12):804-810. |
| APA | Wang, Tingting.,Ma, Junfeng.,Wu, Shuaibin.,Sun, Liangliang.,Yuan, Huiming.,...&Zhang, Yukui.(2011).On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(11-12),804-810. |
| MLA | Wang, Tingting,et al."On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.11-12(2011):804-810. |
入库方式： OAI收割
来源：大连化学物理研究所
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