Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics
文献类型：期刊论文
| 作者 | Sun, Liangliang1,2; Tao, Dingyin1,2; Han, Bin1; Ma, Junfeng1; Zhu, Guijie1; Liang, Zhen1; Shan, Yichu1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 399期号:10页码:3387-3397 |
| 关键词 | Ionic liquid 1-Butyl-3-methyl imidazolium tetrafluoroborate Integral membrane proteins Shotgun |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics |
| 英文摘要 | the solubility and digestion efficiency are two crucial factors that might affect the identification of integral membrane proteins (imps). in this work, 1% (v/v) ionic liquid (il), 1-butyl-3-methyl imidazolium tetrafluoroborate (bmim bf(4)), added in nh(4)hco(3) buffer (ph 8.3), was applied as a sample preparation buffer for imps analysis. compared to the commonly used sodium dodecyl sulfate and methanol methods, the number of identified imps from rat brain by microcolumn reversed phase liquid chromatography (mu rplc)-electrospray ionization tandem mass spectrometry (esi-ms/ms) was improved by over three times, which might be due to the fact that bmim bf(4) offered high solubilizing ability for imps and good compatibility for tryptic digestion. furthermore, compared to rapigest and urea methods, with bmim bf(4) method, the number of identified imps from rat brain could be improved 25% and 80%, respectively, which might be contributed to the good solubilizing ability and high thermal stability of such il. with the sample treated by bmim bf(4) method, by 2d-nanoscx-rplc-esi-ms/ms, 1,450 non-redundant proteins and 7,978 unique peptides were identified from rat brain, and 418 proteins contained at least one predicted transmembrane domain, with false discovery rates of less than 1% for peptide identification, and at least two identified unique peptides per protein. all these results demonstrate that the bmim bf(4) method is of high potential for the large-scale identification of imps. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | desorption/ionization mass-spectrometry ; lc-ms/ms ; trypsin digestion ; plasma-membrane ; large-scale ; proteins ; extraction ; amino ; ph ; identification |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288398700008 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115311]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Liangliang,Tao, Dingyin,Han, Bin,et al. Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics[J]. analytical and bioanalytical chemistry,2011,399(10):3387-3397. |
| APA | Sun, Liangliang.,Tao, Dingyin.,Han, Bin.,Ma, Junfeng.,Zhu, Guijie.,...&Zhang, Yukui.(2011).Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics.analytical and bioanalytical chemistry,399(10),3387-3397. |
| MLA | Sun, Liangliang,et al."Ionic liquid 1-butyl-3-methyl imidazolium tetrafluoroborate for shotgun membrane proteomics".analytical and bioanalytical chemistry 399.10(2011):3387-3397. |
入库方式： OAI收割
来源：大连化学物理研究所
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