Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity
文献类型：期刊论文
| 作者 | Zhang, Liyuan1,2; Xu, Jin3; Sun, Liangliang1,2; Ma, Junfeng1,2; Yang, Kaiguang1; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | analytical and bioanalytical chemistry
 |
| 出版日期 | 2011-04-01 |
| 卷号 | 399期号:10页码:3399-3405 |
| 关键词 | ZrO(2) aerogel Phosphopeptide Enrichment Mass spectrometry |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity |
| 英文摘要 | in this study, zirconium oxide (zro(2)) aerogel was synthesized via a green sol-gel approach, with zirconium oxychloride, instead of the commonly used alkoxide with high toxicity, as the precursor. with such material, phosphopeptides from the digests of 4 pmol of beta-casein with the coexistence of 100 times (mol ratio) bsa could be selectively captured, and identified by maldi-tof ms. due to the large surface area (416.0 m(2) g(-1)) and the mesoporous structure (the average pore size of 10.2 nm) of zro(2) aerogel, a 20-fold higher loading capacity for phosphopeptide, ykvpqleivpn[ps]aeer (mw 1952.12), was obtained compared to that of commercial zro(2) microspheres (341.5 vs. 17.87 mg g(-1)). the metal oxide aerogel was further applied in the enrichment of phosphopeptides from 100 ng nonfat milk, and 17 phosphopeptides were positively identified, with a 1.5-fold improvement in phosphopeptide detection compared with previously reported results. these results demonstrate that zro(2) aerogel can be a powerful enrichment material for phosphoproteome study. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | ion affinity-chromatography ; tin dioxide microspheres ; phosphorylated peptides ; mass-spectrometry ; phosphoproteome analysis ; nanoparticles ; performance ; adsorbent ; growth ; carbon |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000288398700009 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115312]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.China Med Univ, Shengjing Hosp, Dept Hepat Biliary Pancreas & Transplantat Surg, Shenyang 110004, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Liyuan,Xu, Jin,Sun, Liangliang,et al. Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity[J]. analytical and bioanalytical chemistry,2011,399(10):3399-3405. |
| APA | Zhang, Liyuan.,Xu, Jin.,Sun, Liangliang.,Ma, Junfeng.,Yang, Kaiguang.,...&Zhang, Yukui.(2011).Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity.analytical and bioanalytical chemistry,399(10),3399-3405. |
| MLA | Zhang, Liyuan,et al."Zirconium oxide aerogel for effective enrichment of phosphopeptides with high binding capacity".analytical and bioanalytical chemistry 399.10(2011):3399-3405. |
入库方式： OAI收割
来源：大连化学物理研究所
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