Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry
文献类型：期刊论文
| 作者 | Wang, Tingting1,2; Fekete, Agnes3; Gaspar, Andras3; Ma, Junfeng1; Liang, Zhen1; Yuan, Huiming1; Zhang, Lihua1; Schmitt-Kopplin, Philippe3; Zhang, Yukui1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 34期号:4页码:422-427 |
| 关键词 | Capillary isoelectric focusing Low molecular weight acids Mass spectrometry Monolithic immobilized pH gradient |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | analysis of low molecular weight acids by monolithic immobilized ph gradient-based capillary isoelectric focusing coupled with mass spectrometry |
| 英文摘要 | a novel method for the separation and detection of low molecular weight (lmw) acids was developed using monolithic immobilized ph gradient-based capillary isoelectric focusing coupled with mass spectrometry. two main parameters, focusing conditions and delivery buffer conditions, which might affect separation efficiency, were optimized with the focusing time of 7 min at 350 v/cm and the delivery buffer of 50% (v/v) acetonitrile in 10 mmol/l ammonium formate (ph 3.0). under these conditions, the linear correlation between the volume of delivery solvent and the pk(a) of the model components was observed. in addition, the separation mechanism of lmw acids was proposed as well. we suppose that this method may provide a useful tool for the characterization of lmw components (e.g. natural organic matter of different origins). |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | phase liquid-chromatography ; zone-electrophoresis ; protein-analysis ; online ; markers ; points ; device ; column |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287942000008 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115316]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China 3.German Res Ctr Environm Hlth, Helmholtz Zentrum Muenchen, Inst Ecol Chem, Neuherberg, Germany |
| 推荐引用方式 GB/T 7714 | Wang, Tingting,Fekete, Agnes,Gaspar, Andras,et al. Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry[J]. journal of separation science,2011,34(4):422-427. |
| APA | Wang, Tingting.,Fekete, Agnes.,Gaspar, Andras.,Ma, Junfeng.,Liang, Zhen.,...&Zhang, Yukui.(2011).Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry.journal of separation science,34(4),422-427. |
| MLA | Wang, Tingting,et al."Analysis of low molecular weight acids by monolithic immobilized pH gradient-based capillary isoelectric focusing coupled with mass spectrometry".journal of separation science 34.4(2011):422-427. |
入库方式： OAI收割
来源：大连化学物理研究所
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