A novel organic-inorganic hybrid monolith for trypsin immobilization
文献类型：期刊论文
| 作者 | Wu ShuaiBin1,2; Ma JunFeng1,2; Yang KaiGuang1; Liu JinXiang1,2; Liang Zhen1; Zhang LiHua1; Zhang YuKui1 |
| 刊名 | science china-life sciences
 |
| 出版日期 | 2011 |
| 卷号 | 54期号:1页码:54-59 |
| 关键词 | immobilized enzyme reactor organic-inorganic hybrid monolith protein identification HPLC-MS/MS |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | a novel organic-inorganic hybrid monolith for trypsin immobilization |
| 英文摘要 | in proteomics, attention has focused on various immobilized enzyme reactors (imers) for the realization of high throughput digestion. in this report, a novel organic-inorganic hybrid monolith based imer was prepared in a 100 mu m i.d. capillary with 3-glycidoxypropyltrimethoxysilane (glymo) as the monomer and tetraethoxysilane (teos) as the crosslinker. trypsin immobilization was achieved via the reaction between vicinal diol groups, which were obtained from hydrolysis of epoxy groups, and the amino groups of trypsin. bovine serum albumin was digested thoroughly by this imer in 47 s. after micro-reverse phase liquid chromatography-tandem mass spectrometry (mu rplc-ms/ms) analysis and database searching, beyond 35% sequence coverage was obtained, and the result was comparable to that of 12 h in solution digestion. the present imer has potential for high throughput digestion. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biology |
| 研究领域[WOS] | life sciences & biomedicine - other topics |
| 关键词[WOS] | protein digestion ; identification ; system ; proteolysis ; reactor ; online |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000286470400008 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115318]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu ShuaiBin,Ma JunFeng,Yang KaiGuang,et al. A novel organic-inorganic hybrid monolith for trypsin immobilization[J]. science china-life sciences,2011,54(1):54-59. |
| APA | Wu ShuaiBin.,Ma JunFeng.,Yang KaiGuang.,Liu JinXiang.,Liang Zhen.,...&Zhang YuKui.(2011).A novel organic-inorganic hybrid monolith for trypsin immobilization.science china-life sciences,54(1),54-59. |
| MLA | Wu ShuaiBin,et al."A novel organic-inorganic hybrid monolith for trypsin immobilization".science china-life sciences 54.1(2011):54-59. |
入库方式： OAI收割
来源：大连化学物理研究所
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