Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins
文献类型：期刊论文
| 作者 | Gai, Qingqing1; Qu, Feng1; Zhang, Tao1; Zhang, Yukui2 |
| 刊名 | talanta
 |
| 出版日期 | 2011-07-15 |
| 卷号 | 85期号:1页码:304-309 |
| 关键词 | Carboxyl modified magnetic particles Aqueous two-phase extraction Protein Magnetic separation |
| ISSN号 | 待补充 |
| 产权排序 | 2,4 |
| 通讯作者 | 屈锋 ; 张玉奎 |
| 中文摘要 | integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins |
| 英文摘要 | both of the magnetic particle adsorption and aqueous two-phase extraction (atpe) were simple, fast and low-cost method for protein separation. selective proteins adsorption by carboxyl modified magnetic particles was investigated according to protein isoelectric point, solution ph and ionic strength. aqueous two-phase system of peg/sulphate exhibited selective separation and extraction for proteins before and after magnetic adsorption. the two combination ways, magnetic adsorption followed by atpe and atpe followed by magnetic adsorption, for the separation of proteins mixture of lysozyme, bovine serum albumin, trypsin, cytochrome c and myloglobin were discussed and compared. the way of magnetic adsorption followed by atpe was also applied to human serum separation. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | proteomic analysis ; systems ; purification ; copolymer ; lysozyme ; serum ; beads |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000292848900041 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115324]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gai, Qingqing,Qu, Feng,Zhang, Tao,et al. Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins[J]. talanta,2011,85(1):304-309. |
| APA | Gai, Qingqing,Qu, Feng,Zhang, Tao,&Zhang, Yukui.(2011).Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins.talanta,85(1),304-309. |
| MLA | Gai, Qingqing,et al."Integration of carboxyl modified magnetic particles and aqueous two-phase extraction for selective separation of proteins".talanta 85.1(2011):304-309. |
入库方式： OAI收割
来源：大连化学物理研究所
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