The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation
文献类型：期刊论文
| 作者 | Gai, Qing-Qing1; Qu, Feng1; Zhang, Tao1; Zhang, Yu-Kui2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-06-03 |
| 卷号 | 1218期号:22页码:3489-3495 |
| 关键词 | Molecularly imprinted polymer Magnetic particles Bovine serum albumin Protein separation |
| ISSN号 | 待补充 |
| 产权排序 | 2,4 |
| 通讯作者 | 屈锋 ; 张玉奎 |
| 中文摘要 | the preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation |
| 英文摘要 | currently, small proteins imprinting are more reported since large proteins molecular imprinting faces challenge due to their bulk size and complex structure. in this work, bovine serum albumin (bsa) surface-imprinted magnetic polymer was successfully synthesized based on atomic transfer radical polymerization (atrp) method in the presence of common monomer (n-isopropylacrylamide) with the assistant of basic functional monomer (n-[3-(dimethylamino)propyl]-methacrylamide), which provides a achievable attempt for imprinting larger target proteins based on the atpr with the mild reaction conditions. the bsa-imprinted polymer exhibited higher adsorption capacity and selectivity to bsa over the non-imprinted polymer. competitive adsorption tests indicated the bsa-imprinted polymer had better selective adsorption and recognition properties to bsa in the mixture. the obtained bsa-imprinted polymer was applied to bovine serum, which also showed selectivity to bsa. in addition, a conventional aqueous two-phase solution of peg/sulphate was used as elution for adsorbed bsa, which was compared with common nacl elution. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | submicrometer particles ; magnetic-susceptibility ; recognition property ; aqueous-media ; hydrogel ; microspheres ; lysozyme ; beads |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291132600011 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115325]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Beijing Inst Technol, Sch Life Sci, Beijing 100081, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gai, Qing-Qing,Qu, Feng,Zhang, Tao,et al. The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation[J]. journal of chromatography a,2011,1218(22):3489-3495. |
| APA | Gai, Qing-Qing,Qu, Feng,Zhang, Tao,&Zhang, Yu-Kui.(2011).The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation.journal of chromatography a,1218(22),3489-3495. |
| MLA | Gai, Qing-Qing,et al."The preparation of bovine serum albumin surface-imprinted superparamagnetic polymer with the assistance of basic functional monomer and its application for protein separation".journal of chromatography a 1218.22(2011):3489-3495. |
入库方式： OAI收割
来源：大连化学物理研究所
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