A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein
文献类型：期刊论文
| 作者 | Qin, Lei1; He, Xi-Wen1; Jia, Man1; Li, Wen-You1; Zhang, Yu-Kui1,2 |
| 刊名 | chemistry-a european journal
 |
| 出版日期 | 2011 |
| 卷号 | 17期号:5页码:1696-1704 |
| 关键词 | antigens imprinting monolith columns proteins thermosensitivity |
| ISSN号 | 待补充 |
| 产权排序 | 2,5 |
| 通讯作者 | 张玉奎 |
| 中文摘要 | a thermosensitive monolithic column as an artificial antibody for the on-line selective separation of the protein |
| 英文摘要 | the main objective of this study was to develop a new methodology for the preparation of a protein (antigen) that is a molecularly imprinted polymer (mip, an artificial antibody) modified onto the surface of a silica skeleton in which the resulting stationary phase is thermosensitive. the silica monolithic skeleton with vinyl groups was synthesized in a stainless-steel column by using a mild one-step sol gel process with two types of precursor: methyltrimethoxysilane (mtms) and gamma-methacryloxypropyltrimethoxysilane (gamma-maps). subsequently, three types of the thermosensitive protein mip were anchored onto the surface of the silica skeleton to prepare the mip monoliths, which were systematically investigated for back pressure and separation ability at different temperatures to establish good imprinting conditions. under the optimized imprinting conditions, the chromatographic behavior of the thermosensitive mip monolith exhibited strong retention ability for the lysozyme template (target antigen) in relation to the non-imprinting monolith (nip monolith). the imprinting factor (if) for lysozyme reached 3.48 at 20 c. moreover, this new type of artificial antibody displayed favorable binding characteristics for lysozyme over competitive proteins and was further evaluated to selectively separate lysozyme in a real sample by using an on-line method. the run-to-run and column-to-column repeatability measurements of the thermosensitive mip monoliths were also satisfactory. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | molecularly imprinted polymers ; liquid-chromatography ; responsive polymer ; recognition ; gels ; adsorption ; silica ; temperature ; hemoglobin ; hydrogels |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287555600035 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115331]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Nankai Univ, Dept Chem, Tianjin 300071, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qin, Lei,He, Xi-Wen,Jia, Man,et al. A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein[J]. chemistry-a european journal,2011,17(5):1696-1704. |
| APA | Qin, Lei,He, Xi-Wen,Jia, Man,Li, Wen-You,&Zhang, Yu-Kui.(2011).A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein.chemistry-a european journal,17(5),1696-1704. |
| MLA | Qin, Lei,et al."A Thermosensitive Monolithic Column as an Artificial Antibody for the On-line Selective Separation of the Protein".chemistry-a european journal 17.5(2011):1696-1704. |
入库方式： OAI收割
来源：大连化学物理研究所
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