Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis
文献类型：期刊论文
| 作者 | Liang, Yu1,2; Tao, Dingyin1,2; Ma, Junfeng1; Sun, Liangliang1,2; Liang, Zhen1; Zhang, Lihua1; Zhang, Yukui1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-05-20 |
| 卷号 | 1218期号:20页码:2898-2905 |
| 关键词 | Immobilized enzyme reactor Hydrophilic monolith Capillary Microchip Proteomic analysis |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 张丽华 |
| 中文摘要 | hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis |
| 英文摘要 | a novel kind of hydrophilic monolith based immobilized enzyme reactors (imers) was prepared both in uv-transparent capillaries and on glass microchips by the photopolymerization of n-acryloxysuccinimide and poly(ethylene glycol)diacrylate, followed by trypsin immobilization. the performance of capillary imers for protein digestion was evaluated by the digestion of myoglobin with the residential time from 12s to 71 s. with mu rplc-esi-ms/ms analysis, the obtained sequence coverages were all over 80%, comparable to that obtained by in-solution digestion for 12 h. the nonspecific absorption of bsa on monolithic support was evaluated, and no obvious protein residue was observed by a fluorescence assay. moreover, no carry-over of the digests on the capillary imer was found after the digestion of myoglobin (24 mu g) and bsa (9 mu g), which further demonstrated the good hydrophilicity of such matrix. in addition, an integrated microchip-based system involving on-line protein digestion by microchip-based imer, peptides separation by nanorplc and identification by esi-ms/ms was established, by which a mixture of standard proteins and one rplc fraction of escherichia coli extract were successfully identified, indicating that the hydrophilic monolith based imer might provide a promising tool for high-throughput proteomic analysis. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | porous polymer monoliths ; mass-spectrometry ; protein identification ; electrospray-ionization ; liquid-chromatography ; sol-gel ; trypsin ; separation ; digestion ; chip |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291065500010 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115332]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Yu,Tao, Dingyin,Ma, Junfeng,et al. Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis[J]. journal of chromatography a,2011,1218(20):2898-2905. |
| APA | Liang, Yu.,Tao, Dingyin.,Ma, Junfeng.,Sun, Liangliang.,Liang, Zhen.,...&Zhang, Yukui.(2011).Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis.journal of chromatography a,1218(20),2898-2905. |
| MLA | Liang, Yu,et al."Hydrophilic monolith based immobilized enzyme reactors in capillary and on microchip for high-throughput proteomic analysis".journal of chromatography a 1218.20(2011):2898-2905. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。