Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction
文献类型：期刊论文
| 作者 | Li Cun-man2; Xiao Yuan-sheng1; Xue Xing-ya1; Feng Jia-tao1; Zhang Xiu-li1; Liang Xin-miao1 |
| 刊名 | chemical research in chinese universities
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 27期号:3页码:392-396 |
| 关键词 | Unstable compound Isolation and purification Preparative high performance liquid chromatography Solid-phase extraction Iridoid glucoside |
| ISSN号 | 待补充 |
| 产权排序 | 1,2 |
| 通讯作者 | 张秀莉 ; 梁鑫淼 |
| 中文摘要 | isolation and purification of unstable iridoid glucosides from traditional chinese medicine by preparative high performance liquid chromatography coupled with solid-phase extraction |
| 英文摘要 | an efficient preparative method was successfully developed for isolation and purification of unstable components from medicinal plant extracts, using a combined method of preparative high performance liquid chromatography(hplc) and solid-phase extraction(spe). the aim of this study was to obtain an effective method with high preparative efficiency and importantly to avoid the transformation of unstable compounds. the preparative hplc system was based on an lc/ms controlled four-channel autopurification system. the spe method was performed with a c(18) packing material to trap the target compounds and to remove the acidic additive derived from the mobile phase. using this method, the unstable iridoid glucosides(igs) as model compounds were successfully isolated and purified from the extract of hedyotis diffusa willd. six igs(including one new minor ig) and one nucleotide compound were simultaneously obtained, each with a purity of > 91% as determined by hplc. the structures of the isolated compounds were identified by uplc/q-tof ms, uv, 1d and/or 2d nmr. it was demonstrated that the combination of preparative hplc with spe is a versatile tool for preparative purification of unstable compounds from complex natural products. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, multidisciplinary |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | mass-spectrometry ; monoterpene glucosides ; natural-products ; fractionation ; ionization ; glycosides ; diffusa |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291505100011 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115343]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Hebei Univ Sci & Technol, Hebei Res Ctr Anal & Testing, Shijiazhuang 050018, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li Cun-man,Xiao Yuan-sheng,Xue Xing-ya,et al. Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction[J]. chemical research in chinese universities,2011,27(3):392-396. |
| APA | Li Cun-man,Xiao Yuan-sheng,Xue Xing-ya,Feng Jia-tao,Zhang Xiu-li,&Liang Xin-miao.(2011).Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction.chemical research in chinese universities,27(3),392-396. |
| MLA | Li Cun-man,et al."Isolation and Purification of Unstable Iridoid Glucosides from Traditional Chinese Medicine by Preparative High Performance Liquid Chromatography Coupled with Solid-phase Extraction".chemical research in chinese universities 27.3(2011):392-396. |
入库方式： OAI收割
来源：大连化学物理研究所
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