Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column
文献类型：期刊论文
| 作者 | Feng, Jiatao1; Xiao, Yuansheng1; Guo, Zhimou1; Yu, Danhua2; Jin, Yu2; Liang, Xinmiao1,2 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011-02-01 |
| 卷号 | 34期号:3页码:299-307 |
| 关键词 | Click oligo (ethylene glycol) column C18 column Lignum Dalbergia Odorifera Traditional Chinese medicine Two-dimensional preparation |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 梁鑫淼 |
| 中文摘要 | purification of compounds from lignum dalbergia odorifera using two-dimensional preparative chromatography with click oligo (ethylene glycol) and c18 column |
| 英文摘要 | purification of compounds from traditional chinese medicines (tcms) is an important task for understanding the chemical composition of tcms. however, it is difficult to obtain compounds with high enough purity for identification by nmr due to the complexity of tcms in chemical composition. in this study, a two-dimensional purification method based on a click oligo (ethylene glycol) column and a c18 column was developed to realize an orthogonal separation in preparative level for purifying compounds efficiently. the first dimensional preparation was performed on a click oligo (ethylene glycol) column to simplify the sample into the fractions with good separation repeatability. on the first dimension, 7.2 g sample was separated into 11 fractions with a recovery of 86% within 6 h. a c18 column was taken as the second dimension to realize the high-performance separation and rapid preparation from the fractions collected from the first dimension. eight compounds in fraction 6 and 2 compounds in fraction 8 were isolated and identified after optimizing the separation and collection parameters. this method is a high-efficient and orthogonal preparation method to improve the separation of a complex sample and increase the purity of the compounds, which benefits from the application of novel materials in the preparation and purification. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | counter-current chromatography ; performance liquid-chromatography ; phase ; separation |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287426000009 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115354]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China 2.E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Feng, Jiatao,Xiao, Yuansheng,Guo, Zhimou,et al. Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column[J]. journal of separation science,2011,34(3):299-307. |
| APA | Feng, Jiatao,Xiao, Yuansheng,Guo, Zhimou,Yu, Danhua,Jin, Yu,&Liang, Xinmiao.(2011).Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column.journal of separation science,34(3),299-307. |
| MLA | Feng, Jiatao,et al."Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column".journal of separation science 34.3(2011):299-307. |
入库方式： OAI收割
来源：大连化学物理研究所
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