Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment
文献类型：期刊论文
| 作者 | Wan, Huihui1; Yan, Jingyu1; Yu, Long1; Sheng, Qianying2; Zhang, Xiuli1; Xue, Xingya1; Li, Xiuling1; Liang, Xinmiao1,2 |
| 刊名 | analyst
 |
| 出版日期 | 2011 |
| 卷号 | 136期号:21页码:4422-4430 |
| ISSN号 | 待补充 |
| 通讯作者 | 李秀玲 ; 梁鑫淼 |
| 产权排序 | 1,1 |
| 中文摘要 | zirconia layer coated mesoporous silica microspheres as hilic spe materials for selective glycopeptide enrichment |
| 英文摘要 | characterization of protein glycosylation requires highly specific methods for the enrichment of glycopeptides because of their sub-stoichiometric glycosylation-site occupancy. the hydrophilic affinity based strategy has attracted more attention, owing to its broad glycan specificity, good reproducibility, and compatibility with mass spectrometric (ms) analysis. several polar matrices have emerged for hydrophilic interaction chromatography (hilic) approaches, including sepharose, cellulose, zic-hilic and titania. here, we present the solid-phase extraction (spe) utility of zirconia coated mesoporous silica (zro(2)/mps) microspheres for glycopeptide isolation prior to ms analysis. the high specificity of this spe approach was demonstrated by the enrichment of glycopeptides from the digests of model glycoproteins in hilic mode. zro(2)/mps microspheres show superior selectivity and glycosylation heterogeneity coverage for glycopeptide enrichment to conventional sepharose. furthermore, digested mixtures of the phosphoprotein alpha-casein and igg were also treated with zro(2)/mps hilic spe materials, which exhibited that glycopeptides could be effectively enriched with interference from phosphorylated peptides. |
| 学科主题 | 物理化学 |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | hydrophilic-interaction chromatography ; lectin affinity-chromatography ; n-glycosylated proteins ; mass-spectrometry ; liquid-chromatography ; phosphorylated peptides ; facile synthesis ; glycoproteins ; disease ; sba-15 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000295727900007 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115358]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Dalian, Peoples R China 2.E China Univ Sci & Technol, Minist Educ, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wan, Huihui,Yan, Jingyu,Yu, Long,et al. Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment[J]. analyst,2011,136(21):4422-4430. |
| APA | Wan, Huihui.,Yan, Jingyu.,Yu, Long.,Sheng, Qianying.,Zhang, Xiuli.,...&Liang, Xinmiao.(2011).Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment.analyst,136(21),4422-4430. |
| MLA | Wan, Huihui,et al."Zirconia layer coated mesoporous silica microspheres as HILIC SPE materials for selective glycopeptide enrichment".analyst 136.21(2011):4422-4430. |
入库方式： OAI收割
来源：大连化学物理研究所
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