Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography
文献类型：期刊论文
| 作者 | Zhang, Bo2; Sheng, Qianying3; Li, Xiuling1; Liang, Qi; Yan, Jingyu1; Liang, Xinmiao1 |
| 刊名 | journal of separation science
 |
| 出版日期 | 2011-10-01 |
| 卷号 | 34期号:19页码:2745-2750 |
| 关键词 | C18 fractionation Glycopeptide Hydrophilic interaction chromatography Mass spectrometry TiO(2) enrichment |
| ISSN号 | 待补充 |
| 产权排序 | 3,3 |
| 通讯作者 | 梁鑫淼 |
| 中文摘要 | selective enrichment of glycopeptides for mass spectrometry analysis using c18 fractionation and titanium dioxide chromatography |
| 英文摘要 | comprehensive glycoprotein characterization based on mass spectrometry (ms) is challenging because of low concentration of glycopeptides and suppression effect of abundant non-glycosylated peptides in ms. therefore, it is vital to enrich glycopeptides before ms analysis. a new method was developed to selectively enrich glycopeptides from complex sample by coupling c18 fractionation with titanium dioxide (tio(2)) enrichment. the new method allows to selectively enrich n-linked glycopeptides with various glycan forms and different sequence lengths. compared with single tio(2) method, the established method demonstrated higher glycopeptide selectivity and higher glycosylation heterogeneity coverage. further application of this method to mixture of non-glycosylated protein and glycoprotein digests at different levels reveals the feasibility of enrichment of tryptic glycopeptides from simple proteomics samples. |
| WOS标题词 | science & technology ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | protein glycosylation ; lc-ms ; quantification ; identification ; glycoproteomics ; strategies ; discovery |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000296141200026 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115364]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Huazhong Univ Sci & Technol, Dept Gen Surg, Affiliated Union Hosp, Wuhan, Hubei Province, Peoples R China 3.E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bo,Sheng, Qianying,Li, Xiuling,et al. Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography[J]. journal of separation science,2011,34(19):2745-2750. |
| APA | Zhang, Bo,Sheng, Qianying,Li, Xiuling,Liang, Qi,Yan, Jingyu,&Liang, Xinmiao.(2011).Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography.journal of separation science,34(19),2745-2750. |
| MLA | Zhang, Bo,et al."Selective enrichment of glycopeptides for mass spectrometry analysis using C18 fractionation and titanium dioxide chromatography".journal of separation science 34.19(2011):2745-2750. |
入库方式： OAI收割
来源：大连化学物理研究所
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