Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis
文献类型：期刊论文
| 作者 | Zhang, Biaoming1,2; Cao, Xupeng1; Cheng, Xiaofei4; Wu, Peichun1; Xiao, Tonghu4; Zhang, Wei1,3 |
| 刊名 | biotechnology letters
 |
| 出版日期 | 2011-03-01 |
| 卷号 | 33期号:3页码:545-548 |
| 关键词 | Corallina officinalis Heat treatment Protein purification Vanadium-dependent bromoperoxidase |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | weizhang |
| 中文摘要 | efficient purification with high recovery of vanadium bromoperoxidase from corallina officinalis |
| 英文摘要 | a novel, simple and highly efficient process for purifying vanadium bromoperoxidase from corallina officinalis is reported. the key innovation is adding 0.5 mm sodium orthovanadate to the crude cell extract followed by heating at 70a degrees c for 2 h, by which a 5.4-fold purification with a 100% activity recovery was achieved. combining the heat treatment with ammonium sulfate precipitation and deae-52 column chromatography, the overall yield was 84%, 3.8 times greater than the highest yield previously reported. finally, a specific activity of 310 u/mg, a 27-fold purification of the crude enzyme solution was produced. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | pilulifera |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000287502500014 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115393]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Flinders Univ S Australia, Flinders Ctr Marine Bioproc & Bioprod, Adelaide, SA 5042, Australia 4.Ningbo Univ, Fac Mat Sci & Chem Engn, Ningbo 315211, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,et al. Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis[J]. biotechnology letters,2011,33(3):545-548. |
| APA | Zhang, Biaoming,Cao, Xupeng,Cheng, Xiaofei,Wu, Peichun,Xiao, Tonghu,&Zhang, Wei.(2011).Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis.biotechnology letters,33(3),545-548. |
| MLA | Zhang, Biaoming,et al."Efficient purification with high recovery of vanadium bromoperoxidase from Corallina officinalis".biotechnology letters 33.3(2011):545-548. |
入库方式： OAI收割
来源：大连化学物理研究所
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