Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction
文献类型：期刊论文
| 作者 | Li, Yun1; Xie, Xin1; Lee, Milton L.2; Chen, Jiping1 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-12-02 |
| 卷号 | 1218期号:48页码:8608-8616 |
| 关键词 | Solid phase extraction Capillary liquid chromatography Monolithic C18 Hydrophilic Phenols |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 陈吉平 |
| 中文摘要 | preparation and evaluation of hydrophilic c18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction |
| 英文摘要 | hydrophilic c18 monolithic polymer sorbents were synthesized for use in solid phase extraction (spe) and in capillary liquid chromatography (lc). the approach involved incorporating both hydrophobic and hydrophilic monomers into a monolithic material, by copolymerization of stearyl methacrylate (sma), poly(ethylene glycol) methyl ether methacrylate (pegmema) and ethylene dimethacrylate (edma) in the presence of selected porogens, to produce translucent mesoporous monolithic materials in bulk (spe) or white macroporous monoliths inside fused silica capillary columns (capillary lc). a capillary column containing one of the hydrophilic c18 monoliths (i.e. poly(sma-co-pegmema-co-edma) with 15% (w/w) pegmema) demonstrated nearly 35% reduction in retention of polycyclic aromatic compounds and greater than 40% increase in retention of phenols compared to a hydrophobic cl 8 monolithic column. in addition, the hydrophilic monolith demonstrated significantly improved resolution of phenols. similar monolithic materials prepared in bulk were ground and sieved to obtain 45-65 mu m particles with desired rigidity for spe. to achieve optimum extraction performance for phenols, several parameters, including sample ph and volume, and eluent type and volume, were investigated. under optimized experimental conditions, the method demonstrated good sensitivity (1.6 ng/ml lod) and linearity (r(2) > 0.97 for 10-200 ng/ml). again, incorporation of 15% (w/w) pegmema in the monolith increased the extraction efficiency of phenols in water from approximately 20% to 67-92% compared to a hydrophobic c18 monolithic material. increased wettability of the sorbent by the aqueous sample matrix and the presence of hydrogen-bonding interactions are responsible for the improved retention of polar compounds. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | size-exclusion chromatography ; porous polymer monoliths ; mass-spectrometry ; stationary-phase ; new-generation ; columns ; separation ; electrophoresis ; proteins ; coatings |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297386500006 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115467]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Liaoning, Peoples R China 2.Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84602 USA |
| 推荐引用方式 GB/T 7714 | Li, Yun,Xie, Xin,Lee, Milton L.,et al. Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction[J]. journal of chromatography a,2011,1218(48):8608-8616. |
| APA | Li, Yun,Xie, Xin,Lee, Milton L.,&Chen, Jiping.(2011).Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction.journal of chromatography a,1218(48),8608-8616. |
| MLA | Li, Yun,et al."Preparation and evaluation of hydrophilic C18 monolithic sorbents for enhanced polar compound retention in liquid chromatography and solid phase extraction".journal of chromatography a 1218.48(2011):8608-8616. |
入库方式： OAI收割
来源：大连化学物理研究所
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