Electrochemical DNA biosensor for screening of chlorinated benzene pollutants
文献类型：期刊论文
| 作者 | Wu, Lidong; Lu, Xianbo; Jin, Jing; Zhang, Haijun; Chen, Jiping1 |
| 刊名 | biosensors & bioelectronics
 |
| 出版日期 | 2011-06-15 |
| 卷号 | 26期号:10页码:4040-4045 |
| 关键词 | Chlorinated benzenes Electrochemical oligonucleotide biosensor Screening Toxicity evaluation Environmental monitoring |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 陈吉平 |
| 中文摘要 | electrochemical dna biosensor for screening of chlorinated benzene pollutants |
| 英文摘要 | in this work, an electrochemical dna biosensor based on double-stranded dna modified au electrode (dsdna/au) was proposed for the rapid screening and detection of chlorinated benzenes pollutants, in which redox-active methylene blue (mb) was used to amplify the interaction between dsdna and the target analyte. using hexachlorobenzene (hcb) as a model analyte of chlorinated benzenes, the biosensor demonstrated a linear response with the logarithm of hcb concentrations from 100 pmol l(-1) to 100 nmol l(-1). the obtained detection limit was 30 pmol l(-1), which was remarkably superior to other biosensors. the interaction mechanism of the biosensor with hcb was proposed based on systematical characterization by cyclic voltammetry (cv), differential pulse voltammetry (dry), uv-vis spectrometry and electrochemical quartz crystal microbalance (eqcm). further studies revealed that the biosensor could screen chlorinated benzenes in the presence of 100 fold amount of other co-existing chemicals (ethyl acetate and sodium oxalate, etc.), and the response signal of the biosensors for different chlorinated benzenes was correlative to their respective toxicity. the proposed biosensor proved to be a promising "alarm" tool for rapid screening of chlorinated benzenes in real water samples. crown copyright (c) 2011 published by elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry, analytical ; electrochemistry ; nanoscience & nanotechnology |
| 研究领域[WOS] | biophysics ; biotechnology & applied microbiology ; chemistry ; electrochemistry ; science & technology - other topics |
| 关键词[WOS] | chronic myelogenous leukemia ; hepatitis-b-virus ; methylene-blue ; hybridization indicator ; gold ; monolayers ; transport ; electrode ; toxicity ; qsar |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000291904000010 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115470]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wu, Lidong,Lu, Xianbo,Jin, Jing,et al. Electrochemical DNA biosensor for screening of chlorinated benzene pollutants[J]. biosensors & bioelectronics,2011,26(10):4040-4045. |
| APA | Wu, Lidong,Lu, Xianbo,Jin, Jing,Zhang, Haijun,&Chen, Jiping.(2011).Electrochemical DNA biosensor for screening of chlorinated benzene pollutants.biosensors & bioelectronics,26(10),4040-4045. |
| MLA | Wu, Lidong,et al."Electrochemical DNA biosensor for screening of chlorinated benzene pollutants".biosensors & bioelectronics 26.10(2011):4040-4045. |
入库方式： OAI收割
来源：大连化学物理研究所
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