A bead-based approach for large-scale identification of in vitro kinase substrates
文献类型：期刊论文
| 作者 | Zhang, Manyu; Han, Guanghui; Wang, Chunli; Cheng, Kai; Li, Ruibin; Liu, Hongwei; Wei, Xiaoluan; Ye, Mingliang; Zou, Hanfa |
| 刊名 | proteomics
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 11期号:24页码:4632-4637 |
| 关键词 | Interaction Phosphoproteomics Phosphorylation Protein kinases Technology |
| ISSN号 | 1615-9853 |
| 产权排序 | 1,1 |
| 通讯作者 | 叶明亮 ; 邹汉法 |
| 中文摘要 | a bead-based approach for large-scale identification of in vitro kinase substrates |
| 英文摘要 | deciphering the kinasesubstrate relationship is vital for the study of phosphorylation network. the use of immobilized proteins on protein chip as the library for screening of potential kinase substrates is a tried-and-tested method. however, information on phosphorylation sites is lacking and the creation of the library with proteins of whole proteome by recombinant expression is costly and difficult. in this study, a new solid-phase approach by immobilization of proteins from cell lysate onto beads as a protein library for kinase substrate screening was developed. it was found that consensus phosphorylation sites motif for kinase substrates could be accurately determined and hundreds of in vitro kinase substrates and their phosphorylation sites could be identified by using this method. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology |
| 研究领域[WOS] | biochemistry & molecular biology |
| 关键词[WOS] | phosphoproteome analysis ; global analysis ; phosphorylation ; ck2 ; chromatography ; enrichment ; proteomics ; sites ; liver ; cdk1 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297582900005 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115568]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Natl Chromatog Res & Anal Ctr, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Manyu,Han, Guanghui,Wang, Chunli,et al. A bead-based approach for large-scale identification of in vitro kinase substrates[J]. proteomics,2011,11(24):4632-4637. |
| APA | Zhang, Manyu.,Han, Guanghui.,Wang, Chunli.,Cheng, Kai.,Li, Ruibin.,...&Zou, Hanfa.(2011).A bead-based approach for large-scale identification of in vitro kinase substrates.proteomics,11(24),4632-4637. |
| MLA | Zhang, Manyu,et al."A bead-based approach for large-scale identification of in vitro kinase substrates".proteomics 11.24(2011):4632-4637. |
入库方式： OAI收割
来源：大连化学物理研究所
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