中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy

文献类型:期刊论文

作者Zhou, Yongjin J.1,2; Yang, Fan1,2; Zhang, Sufang1; Tan, Haidong1; Zhao, Zongbao K.1
刊名world journal of microbiology & biotechnology
出版日期2011-12-01
卷号27期号:12页码:2999-3003
关键词Disruption cassettes Gene disruption Restriction-free cloning Disruption efficiency Saccharomyces cerevisiae
ISSN号待补充
产权排序1,1
通讯作者赵宗保
中文摘要efficient gene disruption in saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy
英文摘要here we report an improved method for targeted gene disruption with high efficiency in s. cerevisiae, where the selection markers with long homologous arms are defined by the choice of the primer binding sites at the target locus and the disruption cassettes are constructed by restriction-free (rf) cloning strategy. three genes, sam1, idh1 and idh2, were disrupted with this method and the disruption efficiencies of sam1 was improved several folds with much lower false-positive rates compared to the conventional one-step pcr-based gene disruption method. this approach for gene disruption cassettes construction with long flanking homologous arms may be readily applicable to facilitate targeted gene disruption in other non-conventional yeasts and fungi.
WOS标题词science & technology ; life sciences & biomedicine
学科主题物理化学
类目[WOS]biotechnology & applied microbiology
研究领域[WOS]biotechnology & applied microbiology
关键词[WOS]deletion ; genome
收录类别SCI
语种英语
WOS记录号WOS:000297594400029
公开日期2012-07-09
源URL[http://159.226.238.44/handle/321008/115572]  
专题大连化学物理研究所_中国科学院大连化学物理研究所
作者单位1.CAS, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714
Zhou, Yongjin J.,Yang, Fan,Zhang, Sufang,et al. Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy[J]. world journal of microbiology & biotechnology,2011,27(12):2999-3003.
APA Zhou, Yongjin J.,Yang, Fan,Zhang, Sufang,Tan, Haidong,&Zhao, Zongbao K..(2011).Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy.world journal of microbiology & biotechnology,27(12),2999-3003.
MLA Zhou, Yongjin J.,et al."Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy".world journal of microbiology & biotechnology 27.12(2011):2999-3003.

入库方式: OAI收割

来源:大连化学物理研究所

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