Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy
文献类型：期刊论文
| 作者 | Zhou, Yongjin J.1,2; Yang, Fan1,2; Zhang, Sufang1; Tan, Haidong1; Zhao, Zongbao K.1 |
| 刊名 | world journal of microbiology & biotechnology
 |
| 出版日期 | 2011-12-01 |
| 卷号 | 27期号:12页码:2999-3003 |
| 关键词 | Disruption cassettes Gene disruption Restriction-free cloning Disruption efficiency Saccharomyces cerevisiae |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 赵宗保 |
| 中文摘要 | efficient gene disruption in saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy |
| 英文摘要 | here we report an improved method for targeted gene disruption with high efficiency in s. cerevisiae, where the selection markers with long homologous arms are defined by the choice of the primer binding sites at the target locus and the disruption cassettes are constructed by restriction-free (rf) cloning strategy. three genes, sam1, idh1 and idh2, were disrupted with this method and the disruption efficiencies of sam1 was improved several folds with much lower false-positive rates compared to the conventional one-step pcr-based gene disruption method. this approach for gene disruption cassettes construction with long flanking homologous arms may be readily applicable to facilitate targeted gene disruption in other non-conventional yeasts and fungi. |
| WOS标题词 | science & technology ; life sciences & biomedicine |
| 学科主题 | 物理化学 |
| 类目[WOS] | biotechnology & applied microbiology |
| 研究领域[WOS] | biotechnology & applied microbiology |
| 关键词[WOS] | deletion ; genome |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000297594400029 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115572]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.CAS, Dalian Inst Chem Phys, Div Biotechnol, Dalian 116023, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Yongjin J.,Yang, Fan,Zhang, Sufang,et al. Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy[J]. world journal of microbiology & biotechnology,2011,27(12):2999-3003. |
| APA | Zhou, Yongjin J.,Yang, Fan,Zhang, Sufang,Tan, Haidong,&Zhao, Zongbao K..(2011).Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy.world journal of microbiology & biotechnology,27(12),2999-3003. |
| MLA | Zhou, Yongjin J.,et al."Efficient gene disruption in Saccharomyces cerevisiae using marker cassettes with long homologous arms prepared by the restriction-free cloning strategy".world journal of microbiology & biotechnology 27.12(2011):2999-3003. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。