Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species
文献类型：期刊论文
| 作者 | Gu, Qun1,2; David, Frank2; Lynen, Frederic2; Rumpel, Klaus3; Dugardeyn, Jasper4; Van Der Straeten, Dominique4; Xu, Guowang1; Sandra, Pat2 |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2011-05-27 |
| 卷号 | 1218期号:21页码:3247-3254 |
| 关键词 | Metabolomics Gas chromatography-mass spectrometry Arabidopsis thaliana Multivariate statistical analysis Principal component analysis Mass profiler professional |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 许国旺 ; sandrapat |
| 中文摘要 | evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of arabidopsis species |
| 英文摘要 | in this paper, automated sample preparation, retention time locked gas chromatography-mass spectrometry (gc-ms) and data analysis methods for the metabolomics study were evaluated. a miniaturized and automated derivatisation method using sequential oximation and silylation was applied to a polar extract of 4 types (2 types x 2 ages) of arabidopsis thaliana, a popular model organism often used in plant sciences and genetics. automation of the derivatisation process offers excellent repeatability, and the time between sample preparation and analysis was short and constant, reducing artifact formation. retention time locked (rtl) gas chromatography-mass spectrometry was used, resulting in reproducible retention times and gc-ms profiles. two approaches were used for data analysis. xcms followed by principal component analysis (approach 1) and amdis deconvolution combined with a commercially available program (mass profiler professional) followed by principal component analysis (approach 2) were compared. several features that were up- or down-regulated in the different types were detected. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | gc-ms ; ascorbic-acid ; identification ; metabolites ; senescence ; polyamines ; plants ; onset ; tool |
| 收录类别 | SCI ; ISTP |
| 语种 | 英语 |
| WOS记录号 | WOS:000290883000018 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115671]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China 2.Univ Ghent, Pfizer Analyt Res Ctr, B-9000 Ghent, Belgium 3.Pfizer PharmaTherapeut R&D, Sandwich Labs, Sandwich CT13 9NJ, Kent, England 4.Univ Ghent, Dept Physiol, Lab Funct Plant Biol, B-9000 Ghent, Belgium |
| 推荐引用方式 GB/T 7714 | Gu, Qun,David, Frank,Lynen, Frederic,et al. Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species[J]. journal of chromatography a,2011,1218(21):3247-3254. |
| APA | Gu, Qun.,David, Frank.,Lynen, Frederic.,Rumpel, Klaus.,Dugardeyn, Jasper.,...&Sandra, Pat.(2011).Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species.journal of chromatography a,1218(21),3247-3254. |
| MLA | Gu, Qun,et al."Evaluation of automated sample preparation, retention time locked gas chromatography-mass spectrometry and data analysis methods for the metabolomic study of Arabidopsis species".journal of chromatography a 1218.21(2011):3247-3254. |
入库方式： OAI收割
来源：大连化学物理研究所
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