Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry
文献类型：期刊论文
| 作者 | Huang, Qiang; Yin, Peiyuan; Wang, Jing; Chen, Jing; Kong, Hongwei; Lu, Xin; Xu, Guowang |
| 刊名 | journal of chromatography b-analytical technologies in the biomedical and life sciences
 |
| 出版日期 | 2011-04-15 |
| 卷号 | 879期号:13-14页码:961-967 |
| 关键词 | Metabonomics Liver UPLC-Q-TOF/MS Metabolic profiling Type 2 diabetes |
| ISSN号 | 待补充 |
| 产权排序 | 1,1 |
| 通讯作者 | 许国旺 |
| 中文摘要 | method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry |
| 英文摘要 | a protocol for the metabolic profiling of rat liver was developed based on ultra-performance liquid chromatography coupled to quadrupole time-of-flight mass spectrometry (uplc-q-tof/ms) to explore metabolic state directly. methanol/water (4:1, v:v) was selected as the optimal extraction solvent. the established method was validated with a linearity over the 10-5000 ng/ml for internal standards (is) and got an average correlation coefficient of 0.9986. the intra-day and inter-day rsd for most endogenous compounds were below 15%. and the absolute recovery of is was from 84.8% to 109.1%. liver tissues from diabetic and control rats were enrolled in the subsequent study to show the usefulness of the method. a clear classification between the control and model animals was achieved, some significant metabolites were successfully filtered. these metabolites reflected the abnormal metabolism of diabetic rats. this initial application indicated that the method is suitable and reliable for liver tissue metabolic profiling. it is expected this protocol could also be extended to metabonomic studies of other tissues. (c) 2011 elsevier b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 学科主题 | 物理化学 |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | functional genomics ; uplc-ms ; streptozotocin ; mellitus ; optimization ; spectroscopy ; metabonomics ; samples ; urine ; model |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290005400020 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115680]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Sci Analyt Chem, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Qiang,Yin, Peiyuan,Wang, Jing,et al. Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry[J]. journal of chromatography b-analytical technologies in the biomedical and life sciences,2011,879(13-14):961-967. |
| APA | Huang, Qiang.,Yin, Peiyuan.,Wang, Jing.,Chen, Jing.,Kong, Hongwei.,...&Xu, Guowang.(2011).Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry.journal of chromatography b-analytical technologies in the biomedical and life sciences,879(13-14),961-967. |
| MLA | Huang, Qiang,et al."Method for liver tissue metabolic profiling study and its application in type 2 diabetic rats based on ultra performance liquid chromatography-mass spectrometry".journal of chromatography b-analytical technologies in the biomedical and life sciences 879.13-14(2011):961-967. |
入库方式： OAI收割
来源：大连化学物理研究所
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