A proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro
文献类型：期刊论文
| 作者 | Jiang, Yongkang2; Liu, Hongwei1; Li, Hong3,4; Wang, Fangjun1; Cheng, Kai1; Zhou, Guangdong2,3; Zhang, Wenjie2,3; Ye, Mingliang1; Cao, Yinlin2,3; Liu, Wei2,3 |
| 刊名 | biomaterials
 |
| 出版日期 | 2011-06-01 |
| 卷号 | 32期号:17页码:4085-4095 |
| 关键词 | Mechanical loading Proteomics Human tenocytes Tendon engineering In vitro |
| ISSN号 | 0142-9612 |
| 产权排序 | 2,2 |
| 通讯作者 | weiliu ; 邹汉法 |
| 中文摘要 | a proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro |
| 英文摘要 | previous studies have demonstrated the beneficial effect of mechanical loading on in vitro tendon engineering. to understand the mechanism, human tenocytes and polyglycolic acid long fibers were used for in vitro tendon engineering in a bioreactor system for 12 weeks with and without dynamic loading. the engineered neo-tendons were subjected to proteomic analysis using mass spectrometry along with shotgun strategy. as expected, mechanical loading resulted in a more mature tendon tissue characterized by a firmer tissue texture and densely deposited matrices which formed longitudinally aligned collagen fibers in a highly compact fashion. in contrast, non-loaded neo-tendon revealed loosely and less deposited matrices in a relatively less organized pattern. proteins isolated from two groups of tissues exhibited similar distribution of isoeletric point and molecular weight indicating the similarity and comparability of the tissue specimens. further, proteomic analysis showed that total 758 proteins were identified from both groups with 194 and 177 proteins uniquely presented in loaded and non-loaded tendons, respectively. comparison of loaded and non-loaded tendons revealed 195 significantly up-regulated proteins and 189 significantly down-regulated proteins. the differentially expressed proteins could generally be classified into the categories of extracellular matrix, intra-cellular signaling, cytoskeleton and inflammatory response. among them, significantly up-regulated collagens i and vi, mmp-14, wnt5a, microfilament molecules and some inflammatory factors suggest that the possible mechanism for this particular biological phenomenon may involve increased production of tendon specific matrices, enhanced cross-link of collagens and other matrix molecules, proper matrix remodeling for tissue maturation and mechanotransduction (including non-canonical writ signal pathway) mediated other biological processes. (c) 2011 elsevier ltd. all rights reserved. |
| WOS标题词 | science & technology ; technology |
| 学科主题 | 物理化学 |
| 类目[WOS] | engineering, biomedical ; materials science, biomaterials |
| 研究领域[WOS] | engineering ; materials science |
| 关键词[WOS] | 2-dimensional gel-electrophoresis ; mass-spectrometry ; shotgun proteomics ; trap column ; stem-cells ; protein ; matrix ; identification ; fibroblasts ; repair |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290366600003 |
| 公开日期 | 2012-07-09 |
| 源URL | [http://159.226.238.44/handle/321008/115725]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, CAS Key Lab Separat Analyt Chem, Natl Chromatog R&A Ctr, Dalian 116023, Peoples R China 2.Shanghai Jiao Tong Univ, Sch Med, Shanghai Peoples Hosp 9, Dept Plast & Reconstruct Surg,Shanghai Key Lab Ti, Shanghai 200011, Peoples R China 3.Natl Tissue Engn Ctr China, Shanghai, Peoples R China 4.Hangzhou Dianzi Univ, Coll Life Informat Sci & Instrument Engn, Hangzhou 310018, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | Jiang, Yongkang,Liu, Hongwei,Li, Hong,et al. A proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro[J]. biomaterials,2011,32(17):4085-4095. |
| APA | Jiang, Yongkang.,Liu, Hongwei.,Li, Hong.,Wang, Fangjun.,Cheng, Kai.,...&Zou, Hanfa.(2011).A proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro.biomaterials,32(17),4085-4095. |
| MLA | Jiang, Yongkang,et al."A proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro".biomaterials 32.17(2011):4085-4095. |
入库方式： OAI收割
来源：大连化学物理研究所
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