Adenosine triphosphate-functionalized magnetic nanoparticles with high selectivity for phosphopeptide enrichment
文献类型：会议论文
| 作者 | Zhang LH(张丽华) ; Zhang LY(张丽媛) ; Sun LL(孙良亮) ; Liang Z(梁振) ; Zhang YK(张玉奎) |
| 出版日期 | 2011 |
| 会议名称 | 26th international symposium on microscale bioseparations |
| 会议日期 | 2011-5-1 |
| 会议地点 | 圣地亚哥 |
| 页码 | 66-0 |
| 通讯作者 | 张丽华 |
| 中文摘要 | reversible protein phosphorylation plays pivotal roles in signal transduction, cell cycle, gene expression and enzymatic regulation. however, the low abundance of phosphopeptides and the signal suppression by nonphosphopeptides in ms render the comprehensive characterization of phosphoproteome still a challenge. therefore, the selective enrichment of phosphopeptides prior to ms analysis is indispensable. immobilized metal affinity chromatography (imac) is a popular approach for phosphopeptide enrichment. usually, either iminodiacetic acid (ida) or nitrilotriacetic aicd (nta) was used as the ligand to immobilize metal ions. however, the nonspecific interaction between ligand and non-phosphopeptides might result in decreased enrichment specificity. with phosphate groups as ligands, the selectivity of imac was obviously improved. however, the applied reagents to introduce ligands, such as phosphorus oxychloride and 3-(trihydroxysilyl)propyl methylphosphate, contain only one phosphate group. in addition, such ligands were easy to hydrolysis in air, making the imac material preparation harsh and hard to follow. therefore, exploiting new metal ion immobilization ligands, which could not only ensure improved the binding capacity and specificity for phosphopeptide enrichment, but also with a simple, fast and safe imac material preparation protocol is of great significance. in our recent work, adenosine triphosphate (atp) with triphosphate group and biological affinity was used as the ligand to prepared imac materials. to achieve fast and facile phosphopeptide enrichment, amine functionalized magnetic nanoparticles (mnps) were chosen as the matrix, and atp was coupled to mnps via glutaraldehyde, followed by ti(iv) chelation. by such ti(iv)-atp-mnps, 10 phosphopeptides could be effectively isolated from the digest mixture of α-casein and bsa with the molar ratio as 1:5000. compared with the latest reported on ti(iv) immobilized porous phosphate polymer beads[1], the selectivity was improved by 10 folds, indicating the potential of ti(iv)-atp-mnp in phosphoproteome research. |
| 合作状况 | 墙报 |
| 会议主办者 | casss |
| 会议录 | 26th international symposium on microscale bioseparations
 |
| 会议录出版者 | 待补充 |
| 学科主题 | 分析化学 |
| 会议录出版地 | 待补充 |
| 语种 | 英语 |
| 源URL | [http://159.226.238.44/handle/321008/116023]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang LH,Zhang LY,Sun LL,et al. Adenosine triphosphate-functionalized magnetic nanoparticles with high selectivity for phosphopeptide enrichment[C]. 见:26th international symposium on microscale bioseparations. 圣地亚哥. 2011-5-1. |
入库方式： OAI收割
来源：大连化学物理研究所
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