Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis
文献类型：会议论文
| 作者 | Zhang LH(张丽华) ; Yuan HM(袁辉明) ; Zhou Y(周愿) ; Zhang YK(张玉奎) ; Liang Z(梁振) ; Liang ZC(梁作成) |
| 出版日期 | 2011 |
| 会议名称 | pittsburgh conference on analytical chemistry and applied spectroscopy |
| 会议日期 | 2011-3-13 |
| 会议地点 | 亚特兰大 |
| 页码 | 739-0 |
| 通讯作者 | 张丽华 |
| 中文摘要 | the complexity of proteome brings great challenge and opportunity to promote the development of new platforms for liquid phase based proteome analysis. in our recent study, to achieve high throughput, high accuracy and high sensitivity proteome analysis, 2d-sec-micro rplc for protein separation, a hollow fiber based interface to remove acetonitrile in the eluants of microrplc and adjust the ph to 8.0, immobilized trypsin reactor for on-line protein digestion, and microrplc-esi-ms/ms for peptides separation and identification were integrated, which could be fully automatically controlled with a self-designed software. by such an integrated platform, within 21 hr, 2014 proteins were identified from rat brain with fdr<1%. compared to the widely applied bottom up strategy, not only the analysis throughput could be obviously improved by integrating on-line protein digestion, but also the accuracy for protein identification could be increased by protein fractionation with 2d-hplc. |
| 合作状况 | 分会口头报告 |
| 会议主办者 | 匹兹堡光谱学会 |
| 会议录 | pittcon-the magnet for scientific innovation
 |
| 会议录出版者 | 待补充 |
| 学科主题 | 分析化学 |
| 会议录出版地 | 待补充 |
| 语种 | 英语 |
| 源URL | [http://159.226.238.44/handle/321008/116032]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhang LH,Yuan HM,Zhou Y,et al. Integrated Multidimensional Liquid Chromatography Mass Spectrometry for High Throughput Proteome Anaysis[C]. 见:pittsburgh conference on analytical chemistry and applied spectroscopy. 亚特兰大. 2011-3-13. |
入库方式： OAI收割
来源：大连化学物理研究所
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