Roles of N-acetylglucosaminyltransferase III in Epithelial-to-mesenchymal Transition Induced by TGF-β1
文献类型：会议论文
| 作者 | Xu QS(许青松) ; Gu JG(顾建国) ; Du YG(杜昱光) |
| 出版日期 | 2011 |
| 会议名称 | The 3rd Asian Communications of Glycobiology and Glycotechnology |
| 会议日期 | 2011-10-27 |
| 会议地点 | 上海 |
| 页码 | 71-0 |
| 其他题名 | N-乙酰氨基葡萄糖转移酶III在转化生长因子β1诱导的上皮间质转化中的作用 |
| 通讯作者 | YuguangDu |
| 中文摘要 | The epithelial-to-mesenchymal transition (EMT) plays crucial roles in the embryonic development, wound healing, tissue repair and cancer progression. In the present study, we demonstrate that transforming growth factor β (TGF-β) down-regulated expression of N-acetylglucosaminyltransferase III (GnT-III) during EMT. The treatment with TGF-β1 resulted in a decrease in E-cadherin expression and GnT-III expression as well as its product, the bisected N-glycans, which was confirmed by E4-PHA lectin blot in human MCF-10A and mouse GE11 cells. To understand roles of GnT-III expression in EMT, the MCF-10A cell was stably transfected with GnT-III. Of particular interesting, overexpression of GnT-III partially, but did inhibit EMT induced by TGF-β1, which were confirmed by cell morphological changes of phase-contrast, immunochemical staining patterns of E-cadherin and actin. In addition, GnT-III modified E-cadherin, which contributed to prolong E-cadherin turnover on cell surface examined by biotinylation and pulse-chase experiments. Consistently, GnT-III expression inhibited β-catenin translocation from cell-cell contact into cytoplasm and nucleus. Furthermore, the transwell assay showed that GnT-III expression suppresses TGF-β-induced cell motility. Taken together, these observations are the first to clearly demonstrate that GnT-III plays important roles in EMT, and explain a molecular mechanism for inhibitory effects of GnT-III on cancer metastasis. |
| 合作状况 | 墙报 |
| 会议主办者 | 中国科学院上海有机化学所 |
| 会议录 | Asian Communications of Glycobiology and Glycotechnology
 |
| 会议录出版者 | 待补充 |
| 学科主题 | 生物化学 |
| 会议录出版地 | 待补充 |
| 源URL | [http://159.226.238.44/handle/321008/116087]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 推荐引用方式 GB/T 7714 | Xu QS,Gu JG,Du YG. Roles of N-acetylglucosaminyltransferase III in Epithelial-to-mesenchymal Transition Induced by TGF-β1[C]. 见:The 3rd Asian Communications of Glycobiology and Glycotechnology. 上海. 2011-10-27. |
入库方式： OAI收割
来源：大连化学物理研究所
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