Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida
文献类型：期刊论文
| 作者 | Xue, Chun-Ying1 ; Li, De-Zhu1; Wang, Qing-Zhong2
|
| 刊名 | PLANTA MEDICA
 |
| 出版日期 | 2009-06-01 |
| 卷号 | 75期号:8页码:873-875 |
| 关键词 | traditional Chinese medicinal plant Cimicifuga foetida Ranunculaceae LightCycler real-time polymerase chain reaction fluorescence melting curve analysis |
| ISSN号 | 0032-0943 |
| 通讯作者 | chyxue@yahoo.cn |
| 英文摘要 | DNA sequence analysis of rDNA internal transcribed spacer (ITS) and fluorescence melting curve analysis of LightCycler real-time polymerase chain reaction products were exploited for their applications in the authentication of the traditional Chinese medicinal plant Cimicifuga foetida from four substitutes: C. heracleifolia, C. dahurica, C. acerina, and C. simplex. According to the melting temperature - which is a function of the GC/AT ratio, length, and nucleotide sequences of the amplified product - C. foetida was differentiated from C. heracleifolia, C. dahurica, C. acerina, and C. simplex. Melting curve analysis offers a rapid and reliable method for the authentication of the traditional Chinese medicinal plant C. foetida. |
| 学科主题 | Plant Sciences; Chemistry, Medicinal; Pharmacology & Pharmacy |
| 类目[WOS] | Plant Sciences ; Chemistry, Medicinal ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Plant Sciences ; Pharmacology & Pharmacy |
| 关键词[WOS] | MOLECULAR AUTHENTICATION |
| 收录类别 | SCI |
| 资助信息 | Natural Science Foundation of China [NSFC 30770153] |
| 语种 | 英语 |
| WOS记录号 | WOS:000267668700017 |
| 公开日期 | 2012-07-18 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/13951]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Biodivers & Biogeog, Kunming 650204, Yunnan, Peoples R China 2.Yunnan Univ, Dept Chem, Kunming 650091, Yunnan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xue, Chun-Ying,Li, De-Zhu,Wang, Qing-Zhong. Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida[J]. PLANTA MEDICA,2009,75(8):873-875. |
| APA | Xue, Chun-Ying,Li, De-Zhu,&Wang, Qing-Zhong.(2009).Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida.PLANTA MEDICA,75(8),873-875. |
| MLA | Xue, Chun-Ying,et al."Application of LightCycler Polymerase Chain Reaction and Melting Curve Analysis to the Authentication of the Traditional Chinese Medicinal Plant Cimicifuga foetida".PLANTA MEDICA 75.8(2009):873-875. |
入库方式： OAI收割
来源：昆明植物研究所
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