Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method
文献类型：期刊论文
| 作者 | Yang, Jun-Bo1; Li, Hong-Tao1 ; Li, De-Zhu1,2; Liu, Jie1,3; Gao, Lian-Ming1,2
|
| 刊名 | HORTSCIENCE
 |
| 出版日期 | 2009-12-01 |
| 卷号 | 44期号:7页码:2043-2045 |
| 关键词 | Himalayan yew population genetics linkage disequilibrium |
| ISSN号 | 0018-5345 |
| 通讯作者 | gaolm@mail.kib.ac.cn |
| 英文摘要 | The Himalayan yew, Taxus wallichiana Zucc., is an endangered species with a scatted distribution in the Eastern Himalayas and southwestern China. In the present study, 10 microsatellite markers from the genome of T. wallichiana were developed using the protocol of fast isolation by amplified fragment length polymorphism of sequences containing repeats (FIASCO). Polymorphism of each locus was assessed in 28 samples from four wild populations of the Himalayan yew. The allele number of the microsatellites ranged from two to five with an average of 2.9 per allele. The observed and expected heterozygosity varied from 0.00 to 1.00 and from 0.3818 to 0.7552, respectively. Cross-species amplification in another two yew species showed eight of them holding promise for sister species. Two of the 10 loci (TG126 and TC49) significantly deviated from Hardy-Weinberg expectations. No significant linkage disequilibrium was detected between the comparisons of these loci. These polymorphic microsatellite markers would be useful tools for population genetics studies and assessing genetic variations to establish conservation strategy of this endangered species. |
| 学科主题 | Horticulture |
| 类目[WOS] | Horticulture |
| 研究领域[WOS] | Agriculture |
| 关键词[WOS] | TAXACEAE ; TAXOL ; POPULATIONS ; CHINA ; LOCI |
| 收录类别 | SCI |
| 资助信息 | National Natural Sciences Foundation of China [30700042]; Natural Sciences Foundation of Yunnan [2007C088M]; Kunming Institute of Botany of CAS [540806321211]; GBOWS ; West Light Foundation of The Chinese Academy of Sciences [9223111W1]; Yunnan Province of China [2008PY064] |
| 语种 | 英语 |
| WOS记录号 | WOS:000272751600047 |
| 公开日期 | 2012-07-18 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/14037]  |
| 专题 | 昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室 |
| 作者单位 | 1.Chinese Acad Sci, Germplasm Bank Wild Species, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 2.Chinese Acad Sci, Key Lab Biodivers & Biogeog, Kunming Inst Bot, Kunming 650204, Yunnan, Peoples R China 3.Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,et al. Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method[J]. HORTSCIENCE,2009,44(7):2043-2045. |
| APA | Yang, Jun-Bo,Li, Hong-Tao,Li, De-Zhu,Liu, Jie,&Gao, Lian-Ming.(2009).Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method.HORTSCIENCE,44(7),2043-2045. |
| MLA | Yang, Jun-Bo,et al."Isolation and Characterization of Microsatellite Markers in the Endangered Species Taxus wallichiana Using the FIASCO Method".HORTSCIENCE 44.7(2009):2043-2045. |
入库方式： OAI收割
来源：昆明植物研究所
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