Induction of leukemia cell apoptosis by cheliensisin A involves down-regulation of Bcl-2 expression
文献类型：期刊论文
| 作者 | Zhong, L; Li, CM; Hao, XJ ; Lou, LG
|
| 刊名 | ACTA PHARMACOLOGICA SINICA
 |
| 出版日期 | 2005-05-01 |
| 卷号 | 26期号:5页码:623-628 |
| 关键词 | Goniothalamus cheliensis cheliensisin A apoptosis HL-60 cells bcl-2 genes cyclic AMP-dependent protein kinases caspases |
| 英文摘要 | Aim: To investigate the apoptosis-inducing effect of cheliensisin A (GC-51), a novel styryl-lactone isolated from Goniothalamus cheliensis, on human promyelocytic leukemia HL-60 cells and the mechanism of action involved. Methods: Apoptotic cell death was determined by morphological examination and DNA agarose gel electrophoresis. The activity of caspase-3 was assessed using Western blotting and the expression of Bcl-2 and Bax genes was analyzed using the reverse transcription-polymerase chain reaction (RT-PCR) method. Results: GC-51 significantly inhibited the proliferation of HL-60 cells with an IC50 of 2.4± 0. 2 μ mol/L and effectively induced apoptosis in HL-60 cells. Exposure of HL-60 cells to 10 μ mol/L GC-51 for 8 h resulted in approximately 53% of the cells undergoing apoptosis. Caspase-3 was activated in GC-51-treated cells, which was manifested by the appearance of the 17 kDa active form of caspase-3 and the cleavage of poly(ADP-ribose) polymerase (PARP). Meanwhile, GC-51 markedly reduced the expression of the anti-apoptotic gene Bcl-2 and increased the expression of the pro-apoptotic gene Bax. The apoptosis-inducing effect of GC-51 was cAMP-dependent protein kinase (PKA) dependent because PKA, but not the protein kinase C, specific inhibitor H-89, blocked the induction of apoptosis by GC-51 in HL-60 cells. Conclusion: The results demonstrate that GC-51 effectively induces apoptosis in HL-60 cells and that this effect is PKA-dependent and involves the downregulation of Bcl-2 expression and the activation of caspase-3. |
| 学科主题 | Chemistry, Multidisciplinary; Pharmacology & Pharmacy |
| 类目[WOS] | Chemistry, Multidisciplinary ; Pharmacology & Pharmacy |
| 研究领域[WOS] | Chemistry ; Pharmacology & Pharmacy |
| 关键词[WOS] | PHOSPHORYLATED RAP1B ; CAMP ; GONIOTHALAMIN |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000229103500019 |
| 公开日期 | 2012-07-25 |
| 源URL | [http://ir.kib.ac.cn:8080/handle/151853/14569]  |
| 专题 | 昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室 |
| 作者单位 | 1.Shanghai Inst Biol Sci, Inst Mat Med, Shanghai 201203, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhong, L,Li, CM,Hao, XJ,et al. Induction of leukemia cell apoptosis by cheliensisin A involves down-regulation of Bcl-2 expression[J]. ACTA PHARMACOLOGICA SINICA,2005,26(5):623-628. |
| APA | Zhong, L,Li, CM,Hao, XJ,&Lou, LG.(2005).Induction of leukemia cell apoptosis by cheliensisin A involves down-regulation of Bcl-2 expression.ACTA PHARMACOLOGICA SINICA,26(5),623-628. |
| MLA | Zhong, L,et al."Induction of leukemia cell apoptosis by cheliensisin A involves down-regulation of Bcl-2 expression".ACTA PHARMACOLOGICA SINICA 26.5(2005):623-628. |
入库方式： OAI收割
来源：昆明植物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。