A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning
文献类型：期刊论文
| 作者 | Shao, F; Hu, Z; Xiong, YM; Huang, QZ; Wang, CG; Zhu, RH; Wang, DC |
| 刊名 | BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY
 |
| 出版日期 | 1999-03-19 |
| 卷号 | 1430期号:2页码:262-268 |
| 关键词 | antifungal peptide knottin-like peptide amino acid sequence cDNA cloning |
| ISSN号 | 0167-4838 |
| 通讯作者 | wdc@pewdc.ibp.ac.cn |
| 英文摘要 | An antifungal peptide from seeds of Phytolacca americana, designated PAFP-s, has been isolated. The peptide is highly basic and consists of 38 residues with three disulfide bridges. Its molecular mass of 3929.0 was determined by mass spectrometry. The complete amino acid sequence was obtained from automated Edman degradation, and cDNA cloning was successfully performed by 3'-RACE. The deduced amino acid sequence of a partial cDNA corresponded to the amino acid sequence from chemical sequencing. PAFP-s exhibited a broad spectrum of antifungal activity, and its activities differed among various fungi. PAFP-s displayed no inhibitory activity towards Escherichia coli. PAFP-s shows significant sequence similarities and the same cysteine motif with Mj-AMPs, antimicrobial peptides from seeds of Mirabilis jalapa belonging to the knottin-type antimicrobial peptide. (C) 1999 Elsevier Science B.V. All rights reserved. |
| 学科主题 | Biochemistry & Molecular Biology; Biophysics |
| 类目[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Biophysics |
| 关键词[WOS] | MIRABILIS-JALAPA L ; ANTIMICROBIAL PEPTIDES ; PHYTOPHTHORA-INFESTANS ; INHIBITORY ACTIVITY ; PROTEINS ; BARLEY |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000079411700010 |
| 公开日期 | 2012-07-25 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/14367]  |
| 专题 | 昆明植物研究所_离退休 |
| 作者单位 | 1.Chinese Acad Sci, Inst Biophys, Beijing 100101, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650204, Peoples R China |
| 推荐引用方式 GB/T 7714 | Shao, F,Hu, Z,Xiong, YM,et al. A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning[J]. BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1999,1430(2):262-268. |
| APA | Shao, F.,Hu, Z.,Xiong, YM.,Huang, QZ.,Wang, CG.,...&Wang, DC.(1999).A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning.BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY,1430(2),262-268. |
| MLA | Shao, F,et al."A new antifungal peptide from the seeds of Phytolacca americana: characterization, amino acid sequence and cDNA cloning".BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY 1430.2(1999):262-268. |
入库方式： OAI收割
来源：昆明植物研究所
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