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Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus

文献类型：期刊论文

作者Liu, Yuan1,2; Cui, Zhaoxia1; Luan, Weisha1,2; Song, Chengwen1,2; Nie, Qing1,2; Wang, Shuangyan1,2; Li, Qianqian1,2
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2011-02-01
卷号30期号:2页码:583-591
关键词Portunus trituberculatus Anti-lipopolysaccharide factor SNP Expression analysis
ISSN号1050-4648
中文摘要Anti-lipopolysaccharide factors (ALFs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (LPS) and exhibit broad spectrum antimicrobial activities. In this study, three isoforms of the ALF homologues (PtesALF1-3) were identified from eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA sequences of PtesALF1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. PtesALF1-3 contained two conserved cysteine residues and shared high similarity with other reported ALFs. Predicted tertiary structures of PtesALF2 and 3 containing four beta-strands and three alpha-helix were similar to that described in Limulus polyphemus, while PtesALF1 had only one alpha-helix in its spatial structure. Sequence analysis revealed PtesALF1-3 were encoded by the same genomic locus and generated by alternative splicing of the pre-mRNA. Totally 89 SNPs including 18 in coding region and 71 in noncoding region were detected by direct sequencing of 30 genomic samples. The mRNA expression of PtesALF1 and PtesALF1-3 transcripts was mainly detected in haemocytes but showed different expression pattern in other tissues including hepatopancreas, gill, eyestalk and muscle. After challenge with Vibrio alginolyticus, the temporal expression level of PtesALF1-3 transcripts in haemocytes showed a clear time-dependent response expression pattern with two peaks within the experimental period of 32 h, while PtesALF1 was up-regulated only once with obvious decrease at 6 h and significant increase at 24 h. These results suggest that the PtesALF isoforms have different tissue specificity and might provide multiple protective functions against invading bacteria in P trituberculatus. (C) 2010 Elsevier Ltd. All rights reserved.
英文摘要Anti-lipopolysaccharide factors (ALFs), as the potent antimicrobial peptides, can bind and neutralize lipopolysaccharide (LPS) and exhibit broad spectrum antimicrobial activities. In this study, three isoforms of the ALF homologues (PtesALF1-3) were identified from eyestalk cDNA library of swimming crab Portunus trituberculatus. The full-length cDNA sequences of PtesALF1, 2 and 3 were 1138, 1052 and 1057 bp encoding 92, 108 and 123 amino acids, respectively. PtesALF1-3 contained two conserved cysteine residues and shared high similarity with other reported ALFs. Predicted tertiary structures of PtesALF2 and 3 containing four beta-strands and three alpha-helix were similar to that described in Limulus polyphemus, while PtesALF1 had only one alpha-helix in its spatial structure. Sequence analysis revealed PtesALF1-3 were encoded by the same genomic locus and generated by alternative splicing of the pre-mRNA. Totally 89 SNPs including 18 in coding region and 71 in noncoding region were detected by direct sequencing of 30 genomic samples. The mRNA expression of PtesALF1 and PtesALF1-3 transcripts was mainly detected in haemocytes but showed different expression pattern in other tissues including hepatopancreas, gill, eyestalk and muscle. After challenge with Vibrio alginolyticus, the temporal expression level of PtesALF1-3 transcripts in haemocytes showed a clear time-dependent response expression pattern with two peaks within the experimental period of 32 h, while PtesALF1 was up-regulated only once with obvious decrease at 6 h and significant increase at 24 h. These results suggest that the PtesALF isoforms have different tissue specificity and might provide multiple protective functions against invading bacteria in P trituberculatus. (C) 2010 Elsevier Ltd. All rights reserved.
学科主题Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
收录类别SCI
原文出处10.1016/j.fsi.2010.12.005
语种英语
WOS记录号WOS:000288305300018
公开日期2012-07-03
源URL[http://ir.qdio.ac.cn/handle/337002/11980]  
专题海洋研究所_实验海洋生物学重点实验室
海洋研究所_海洋生物技术研发中心
作者单位1.Chinese Acad Sci, Inst Oceanol, EMBL, Qingdao 266071, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing 100039, Peoples R China
推荐引用方式
GB/T 7714		 Liu, Yuan,Cui, Zhaoxia,Luan, Weisha,et al. Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus[J]. FISH & SHELLFISH IMMUNOLOGY,2011,30(2):583-591.
APA Liu, Yuan.,Cui, Zhaoxia.,Luan, Weisha.,Song, Chengwen.,Nie, Qing.,...&Li, Qianqian.(2011).Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus.FISH & SHELLFISH IMMUNOLOGY,30(2),583-591.
MLA Liu, Yuan,et al."Three isoforms of anti-lipopolysaccharide factor identified from eyestalk cDNA library of swimming crab Portunus trituberculatus".FISH & SHELLFISH IMMUNOLOGY 30.2(2011):583-591.

入库方式： OAI收割

来源：海洋研究所

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