Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production
文献类型：期刊论文
| 作者 | Zhu, Yueming; Men, Yan; Bai, Wei; Li, Xiaobo; Zhang, Lili; Sun, Yuanxia; Ma, Yanhe |
| 刊名 | BIOTECHNOLOGY LETTERS
 |
| 出版日期 | 2012-10-01 |
| 卷号 | 34期号:10页码:1901-1906 |
| 关键词 | Fructose D-Psicose D-Psicose 3-epimerase Ruminococcus Thermostability |
| 英文摘要 | The d-psicose 3-epimerase (DPE) gene from Ruminococcus sp. was cloned and overexpressed in Escherichia coli. The recombinant protein was purified and characterized. It was optimally active at pH 7.5-8.0 and 60 A degrees C. Activity was not dependent on the presence of metal ions; however, it became more thermostable with added Mn2+. The K (m) of the enzyme for d-psicose (48 mM) was lower than that for d-tagatose (230 mM), suggesting that d-psicose is the optimum substrate. More importantly, the thermostability of the novel DPE from Ruminococcus is the strongest among all of the d-psicose and d-tagatose 3-epimerases and may be suitable for the industrial production of d-psicose from fructose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | D-TAGATOSE 3-EPIMERASE ; PSEUDOMONAS CICHORII ST-24 ; D-FRUCTOSE ; AGROBACTERIUM-TUMEFACIENS ; CLONING ; ENZYME |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000310429800019 |
| 公开日期 | 2012-08-29 |
| 源URL | [http://localhost/handle/0/260]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Natl Engn Lab Ind Enzymes, Tianjin Airport Econ Area, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Yueming,Men, Yan,Bai, Wei,et al. Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production[J]. BIOTECHNOLOGY LETTERS,2012,34(10):1901-1906. |
| APA | Zhu, Yueming.,Men, Yan.,Bai, Wei.,Li, Xiaobo.,Zhang, Lili.,...&Ma, Yanhe.(2012).Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production.BIOTECHNOLOGY LETTERS,34(10),1901-1906. |
| MLA | Zhu, Yueming,et al."Overexpression of D-psicose 3-epimerase from Ruminococcus sp. in Escherichia coli and its potential application in D-psicose production".BIOTECHNOLOGY LETTERS 34.10(2012):1901-1906. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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