Identification and characterization of a novel l-arabinose isomerase from Anoxybacillus flavithermus useful in d-tagatose production
文献类型：期刊论文
| 作者 | Li, Yanjun2; Zhu, Yueming1; Liu, Anjun2; Sun, Yuanxia1 |
| 刊名 | EXTREMOPHILES
 |
| 出版日期 | 2011-05-01 |
| 卷号 | 15期号:3页码:441-450 |
| 关键词 | D-tagatose L-arabinose isomerase Anoxybacillus flavithermus Thermophilic Alkaliphilic enzyme |
| 英文摘要 | d-Tagatose is a highly functional rare ketohexose and many attempts have been made to convert d-galactose into the valuable d-tagatose using l-arabinose isomerase (l-AI). In this study, a thermophilic strain possessing l-AI gene was isolated from hot spring sludge and identified as Anoxybacillus flavithermus based on its physio-biochemical characterization and phylogenetic analysis of its 16s rRNA gene. Furthermore, the gene encoding l-AI from A. flavithermus (AFAI) was cloned and expressed at a high level in E. coli BL21(DE3). l-AI had a molecular weight of 55,876 Da, an optimum pH of 10.5 and temperature of 95A degrees C. The results showed that the conversion equilibrium shifted to more d-tagatose from d-galactose by raising the reaction temperatures and adding borate. A 60% conversion of d-galactose to d-tagatose was observed at an isomerization temperature of 95A degrees C with borate. The catalytic efficiency (k (cat) /K (m)) for d-galactose with borate was 9.47 mM(-1) min(-1), twice as much as that without borate. Our results indicate that AFAI is a novel hyperthermophilic and alkaliphilic isomerase with a higher catalytic efficiency for d-galactose, suggesting its great potential for producing d-tagatose. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 研究领域[WOS] | Biochemistry & Molecular Biology ; Microbiology |
| 关键词[WOS] | D-GALACTOSE ; MYCOBACTERIUM-SMEGMATIS ; D-GALACTITOL ; SP NOV. ; CLONING ; STRAIN ; ACID ; BIOCONVERSION ; PURIFICATION ; EXPRESSION |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000290037700014 |
| 公开日期 | 2011-08-17 |
| 源URL | [http://localhost/handle/0/86]  |
| 专题 | 天津工业生物技术研究所_功能糖与天然活性物质研究组 孙媛霞 _期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China 2.Tianjin Univ Sci & Technol, Coll Food Engn & Biotechnol, Tianjin 300457, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yanjun,Zhu, Yueming,Liu, Anjun,et al. Identification and characterization of a novel l-arabinose isomerase from Anoxybacillus flavithermus useful in d-tagatose production[J]. EXTREMOPHILES,2011,15(3):441-450. |
| APA | Li, Yanjun,Zhu, Yueming,Liu, Anjun,&Sun, Yuanxia.(2011).Identification and characterization of a novel l-arabinose isomerase from Anoxybacillus flavithermus useful in d-tagatose production.EXTREMOPHILES,15(3),441-450. |
| MLA | Li, Yanjun,et al."Identification and characterization of a novel l-arabinose isomerase from Anoxybacillus flavithermus useful in d-tagatose production".EXTREMOPHILES 15.3(2011):441-450. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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