Error correction in gene synthesis technology
文献类型：期刊论文
| 作者 | Ma, Siying1,2; Saaem, Ishtiaq1,2,3; Tian, Jingdong1,2,3 |
| 刊名 | TRENDS IN BIOTECHNOLOGY
 |
| 出版日期 | 2012-03-01 |
| 卷号 | 30期号:3页码:147-154 |
| 英文摘要 | Accurate, economical and high-throughput gene and genome synthesis is essential to the development of synthetic biology and biotechnology. New large-scale gene synthesis methods harnessing the power of DNA microchips have recently been demonstrated. Yet, the technology is still compromised by a high occurrence of errors in the synthesized products. These errors still require substantial effort to correct. To solve this bottleneck, novel approaches based on new chemistry, enzymology or next generation sequencing have emerged. This review discusses these new trends and promising strategies of error filtration, correction and prevention in de novo gene and genome synthesis. Continued innovation in error correction technologies will enable affordable and large-scale gene and genome synthesis in the near future. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biotechnology & Applied Microbiology |
| 研究领域[WOS] | Biotechnology & Applied Microbiology |
| 关键词[WOS] | DNA MISMATCH REPAIR ; STRAND-SPECIFIC NUCLEASES ; INDUCED POINT MUTATIONS ; T4 ENDONUCLEASE-VII ; HIGH-FIDELITY ; CHEMICAL-SYNTHESIS ; CEL-I ; SURVEYOR(TM) NUCLEASE ; RAPID IDENTIFICATION ; MOLECULAR-MECHANISMS |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301277800004 |
| 公开日期 | 2012-05-21 |
| 源URL | [http://localhost/handle/0/221]  |
| 专题 | 天津工业生物技术研究所_合成基因组学实验室 田敬东 _期刊论文 |
| 作者单位 | 1.Duke Univ, Dept Biomed Engn, Durham, NC 27708 USA 2.Duke Univ, Inst Genome Sci & Policy, Durham, NC 27708 USA 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Tianjin 300308, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Siying,Saaem, Ishtiaq,Tian, Jingdong. Error correction in gene synthesis technology[J]. TRENDS IN BIOTECHNOLOGY,2012,30(3):147-154. |
| APA | Ma, Siying,Saaem, Ishtiaq,&Tian, Jingdong.(2012).Error correction in gene synthesis technology.TRENDS IN BIOTECHNOLOGY,30(3),147-154. |
| MLA | Ma, Siying,et al."Error correction in gene synthesis technology".TRENDS IN BIOTECHNOLOGY 30.3(2012):147-154. |
入库方式： OAI收割
来源：天津工业生物技术研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。