PREDICTING SERPIN/PROTEASE INTERACTIONS
文献类型：期刊论文
| 作者 | Song, Jiangning1,2,3; Matthews, Antony Y.1; Reboul, Cyril F.1; Kaiserman, Dion1; Pike, Robert N.1; Bird, Phillip I.1; Whisstock, James C.1,4; Whisstock, JC; Bird, PI |
| 刊名 | METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION
 |
| 出版日期 | 2011 |
| 卷号 | 501期号:501页码:237-273 |
| 英文摘要 | Proteases are tightly regulated by specific inhibitors, such as serpins, which are able to undergo considerable and irreversible conformational changes in order to trap their targets. There has been a considerable effort to investigate serpin structure and functions in the past few decades; however, the specific interactions between proteases and serpins remain elusive. In this chapter, we describe detailed experimental protocols to determine and characterize the extended substrate specificity of proteases based on a substrate phage display technique. We also describe how to employ a bioinformatics system to analyze the substrate specificity data obtained from this technique and predict the potential inhibitory serpin partners of a protease (in this case, the immune protease, granzyme B) in a step-by-step manner. The method described here could also be applied to other proteases for more generalized substrate specificity analysis and substrate discovery. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Biochemical Research Methods ; Biochemistry & Molecular Biology |
| 研究领域[WOS] | Biochemistry & Molecular Biology |
| 关键词[WOS] | SUBSTRATE PHAGE DISPLAY ; SERPIN-PROTEASE COMPLEX ; GRANZYME-B ; CLEAVAGE SITES ; REACTIVE CENTER ; MOLECULAR-BASIS ; SPECIFICITY ; MECHANISM ; THROMBIN ; HEPARIN |
| 收录类别 | BSCI ; SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000301675600012 |
| 公开日期 | 2011-12-28 |
| 源URL | [http://localhost/handle/0/200]  |
| 专题 | 天津工业生物技术研究所_结构生物信息学和整合系统生物学实验室 宋江宁_期刊论文 |
| 作者单位 | 1.Monash Univ, Dept Biochem & Mol Biol, Melbourne, Vic 3004, Australia 2.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, State Engn Lab Ind Enzymes, Tianjin, Peoples R China 3.Chinese Acad Sci, Tianjin Inst Ind Biotechnol, Key Lab Syst Microbial Biotechnol, Tianjin, Peoples R China 4.Monash Univ, ARC Ctr Excellence Struct & Funct Microbial Genom, Clayton, Vic, Australia |
| 推荐引用方式 GB/T 7714 | Song, Jiangning,Matthews, Antony Y.,Reboul, Cyril F.,et al. PREDICTING SERPIN/PROTEASE INTERACTIONS[J]. METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION,2011,501(501):237-273. |
| APA | Song, Jiangning.,Matthews, Antony Y..,Reboul, Cyril F..,Kaiserman, Dion.,Pike, Robert N..,...&Bird, PI.(2011).PREDICTING SERPIN/PROTEASE INTERACTIONS.METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION,501(501),237-273. |
| MLA | Song, Jiangning,et al."PREDICTING SERPIN/PROTEASE INTERACTIONS".METHODS IN ENZYMOLOGY, VOL 501: SERPIN STRUCTURE AND EVOLUTION 501.501(2011):237-273. |
入库方式： OAI收割
来源：天津工业生物技术研究所
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