Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals
文献类型:期刊论文
作者 | Ji, Cheng1,2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1 |
刊名 | TOXICOLOGY AND APPLIED PHARMACOLOGY
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出版日期 | 2012-07-15 |
卷号 | 262期号:2页码:149-155 |
关键词 | Thyroid-stimulating hormone subunit-beta Hypothalamic-pituitary-thyroid Endocrine disrupting Transgenic reporter fish Assessment model |
ISSN号 | 0041-008X |
通讯作者 | Yin, Z (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China. |
中文摘要 | Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved. |
英文摘要 | Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved. |
WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
类目[WOS] | Pharmacology & Pharmacy ; Toxicology |
研究领域[WOS] | Pharmacology & Pharmacy ; Toxicology |
关键词[WOS] | THYROTROPIN-BETA-SUBUNIT ; ENDOCRINE DISRUPTION ; GLAND ; GENE ; EXPRESSION ; PROMOTER ; ACTIVATION ; REGION ; BRAIN |
收录类别 | SCI |
资助信息 | Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012] |
语种 | 英语 |
WOS记录号 | WOS:000305845400006 |
公开日期 | 2012-09-25 |
源URL | [http://ir.ihb.ac.cn/handle/342005/17015] ![]() |
专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
作者单位 | 1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
推荐引用方式 GB/T 7714 | Ji, Cheng,Jin, Xia,He, Jiangyan,et al. Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2012,262(2):149-155. |
APA | Ji, Cheng,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2012).Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals.TOXICOLOGY AND APPLIED PHARMACOLOGY,262(2),149-155. |
MLA | Ji, Cheng,et al."Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals".TOXICOLOGY AND APPLIED PHARMACOLOGY 262.2(2012):149-155. |
入库方式: OAI收割
来源:水生生物研究所
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