中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals

文献类型:期刊论文

作者Ji, Cheng1,2; Jin, Xia1; He, Jiangyan1; Yin, Zhan1
刊名TOXICOLOGY AND APPLIED PHARMACOLOGY
出版日期2012-07-15
卷号262期号:2页码:149-155
关键词Thyroid-stimulating hormone subunit-beta Hypothalamic-pituitary-thyroid Endocrine disrupting Transgenic reporter fish Assessment model
ISSN号0041-008X
通讯作者Yin, Z (reprint author), Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China.
中文摘要Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.
英文摘要Accumulating evidence indicates that a wide range of chemicals have the ability to interfere with the hypothalamic-pituitary-thyroid (HPT) axis. Novel endpoints should be evaluated in addition to existing methods in order to effectively assess the effects of these chemicals on the HPT axis. Thyroid-stimulating hormone subunit beta (TSH beta) plays central regulatory roles in the HPT system. We identified the regulatory region that determines the expression level of zebrafish TSH beta in the anterior pituitary. In the transgenic zebrafish with EGFP driven by the TSH beta promoter, the similar responsive patterns between the expression levels of TSH beta:EGFP and endogenous TSH beta mRNA in the pituitary are observed following treatments with goitrogen chemicals and exogenous thyroid hormones (THs). These results suggest that the TSH beta:EGFP transgenic reporter zebrafish may be a useful alternative in vivo model for the assessment of chemicals interfering with the HPT system. (C) 2012 Elsevier Inc. All rights reserved.
WOS标题词Science & Technology ; Life Sciences & Biomedicine
类目[WOS]Pharmacology & Pharmacy ; Toxicology
研究领域[WOS]Pharmacology & Pharmacy ; Toxicology
关键词[WOS]THYROTROPIN-BETA-SUBUNIT ; ENDOCRINE DISRUPTION ; GLAND ; GENE ; EXPRESSION ; PROMOTER ; ACTIVATION ; REGION ; BRAIN
收录类别SCI
资助信息Hi-Tech Research and Development Program of China[2006AA06Z424]; National Natural Science Foundation of China[2089013, 20977109]; Natural Science Foundation of Hubei Province[2008CDA012]
语种英语
WOS记录号WOS:000305845400006
公开日期2012-09-25
源URL[http://ir.ihb.ac.cn/handle/342005/17015]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
作者单位1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan, Hubei, Peoples R China
2.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Ji, Cheng,Jin, Xia,He, Jiangyan,et al. Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2012,262(2):149-155.
APA Ji, Cheng,Jin, Xia,He, Jiangyan,&Yin, Zhan.(2012).Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals.TOXICOLOGY AND APPLIED PHARMACOLOGY,262(2),149-155.
MLA Ji, Cheng,et al."Use of TSH beta:EGFP transgenic zebrafish as a rapid in vivo model for assessing thyroid-disrupting chemicals".TOXICOLOGY AND APPLIED PHARMACOLOGY 262.2(2012):149-155.

入库方式: OAI收割

来源:水生生物研究所

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