Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)
文献类型：期刊论文
| 作者 | Chen, Lianguo1,2; Hu, Chenyan1; Huang, Changjiang3; Wang, Qiangwei1; Wang, Xiaofang1; Yang, Lihua1; Zhou, Bingsheng1 |
| 刊名 | AQUATIC TOXICOLOGY
 |
| 出版日期 | 2012-09-15 |
| 卷号 | 120页码:11-18 |
| 关键词 | PBDEs Retinoids Retinal Retinyl ester Gene transcription Zebrafish |
| ISSN号 | 0166-445X |
| 通讯作者 | Zhou, BS (reprint author), Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China. |
| 中文摘要 | This study examined the disruptive effect of exposure to polybrominated diphenyl ethers (PBDEs) on retinoid content in zebrafish (Danio rerio). Adult zebrafish were exposed to an environmentally relevant concentration (0.45 mu g/L) and a higher concentration (9.6 mu g/L) of DE-71 for 60 days. Retinoid content and gene transcription levels were examined in female zebrafish. PBDE exposure caused a significant decrease of retinyl ester content in the intestine and a downregulation of intestinal cellular retinal binding protein gene transcription (CRBP1a). In the liver, retinyl ester content was significantly decreased, while retinol content was increased. An upregulation of liver CRBP2a and retinal binding protein (RBP) gene transcription and an increased level of RBP protein were observed. In the eyes, both the retinal and retinyl ester content were increased and CRBPla gene transcription was upregulated. However, the gene encoding for retinal dehydrogenase (1RALDH2), responsible for retinoic acid synthesis, was downregulated in the eyes. CYP26a, the gene responsible for retinoic acid degradation, was upregulated, which indicated an increased level of retinoic acid. In the ovaries, the increased deposition of retinoids was also observed, while gene transcription levels of both CRBPs (CRBP1a and CRBP1b) were upregulated. An increased deposition of retinal was measured in the eggs. Overall, this study demonstrated that long-term exposure of zebrafish to environmentally relevant concentrations of DE-71 disrupted the transport, storage and metabolism of retinoid in various tissues. This study also indicated that retinoid levels in zebrafish are sensitive to PBDE exposure and highlighted the importance of liver storage, which appears to support important functions in reproduction and vision. (C) 2012 Elsevier B.V. All rights reserved. |
| 英文摘要 | This study examined the disruptive effect of exposure to polybrominated diphenyl ethers (PBDEs) on retinoid content in zebrafish (Danio rerio). Adult zebrafish were exposed to an environmentally relevant concentration (0.45 mu g/L) and a higher concentration (9.6 mu g/L) of DE-71 for 60 days. Retinoid content and gene transcription levels were examined in female zebrafish. PBDE exposure caused a significant decrease of retinyl ester content in the intestine and a downregulation of intestinal cellular retinal binding protein gene transcription (CRBP1a). In the liver, retinyl ester content was significantly decreased, while retinol content was increased. An upregulation of liver CRBP2a and retinal binding protein (RBP) gene transcription and an increased level of RBP protein were observed. In the eyes, both the retinal and retinyl ester content were increased and CRBPla gene transcription was upregulated. However, the gene encoding for retinal dehydrogenase (1RALDH2), responsible for retinoic acid synthesis, was downregulated in the eyes. CYP26a, the gene responsible for retinoic acid degradation, was upregulated, which indicated an increased level of retinoic acid. In the ovaries, the increased deposition of retinoids was also observed, while gene transcription levels of both CRBPs (CRBP1a and CRBP1b) were upregulated. An increased deposition of retinal was measured in the eggs. Overall, this study demonstrated that long-term exposure of zebrafish to environmentally relevant concentrations of DE-71 disrupted the transport, storage and metabolism of retinoid in various tissues. This study also indicated that retinoid levels in zebrafish are sensitive to PBDE exposure and highlighted the importance of liver storage, which appears to support important functions in reproduction and vision. (C) 2012 Elsevier B.V. All rights reserved. |
| WOS标题词 | Science & Technology ; Life Sciences & Biomedicine |
| 类目[WOS] | Marine & Freshwater Biology ; Toxicology |
| 研究领域[WOS] | Marine & Freshwater Biology ; Toxicology |
| 关键词[WOS] | POLYBROMINATED DIPHENYL ETHERS ; VITAMIN-A STATUS ; POLYCHLORINATED-BIPHENYLS ; PHOTORECEPTOR DEVELOPMENT ; DECABROMODIPHENYL ETHER ; PIMEPHALES-PROMELAS ; FLAME RETARDANTS ; THYROID-HORMONE ; ACID ; ADULT |
| 收录类别 | SCI |
| 资助信息 | National Natural Science Foundation of China[20890113]; State Key Laboratory of Freshwater Ecology and Biotechnology[Y15B13] |
| 语种 | 英语 |
| WOS记录号 | WOS:000306615500002 |
| 公开日期 | 2012-09-25 |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/16967]  |
| 专题 | 水生生物研究所_水环境工程研究中心_期刊论文 |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Grad Sch, Beijing 100039, Peoples R China 3.Wenzhou Med Coll, Inst Watershed Sci & Environm Ecol, Wenzhou 325035, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Lianguo,Hu, Chenyan,Huang, Changjiang,et al. Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)[J]. AQUATIC TOXICOLOGY,2012,120:11-18. |
| APA | Chen, Lianguo.,Hu, Chenyan.,Huang, Changjiang.,Wang, Qiangwei.,Wang, Xiaofang.,...&Zhou, Bingsheng.(2012).Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio).AQUATIC TOXICOLOGY,120,11-18. |
| MLA | Chen, Lianguo,et al."Alterations in retinoid status after long-term exposure to PBDEs in zebrafish (Danio rerio)".AQUATIC TOXICOLOGY 120(2012):11-18. |
入库方式： OAI收割
来源：水生生物研究所
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